Early events in kidney donation
| Autor: | Theo A. Schuurs, Rutger J. Ploeg, Henri G. D. Leuvenink, Wim van Oeveren, Gerhard Rakhorst, Aurora M. Morariu |
|---|---|
| Přispěvatelé: | Groningen Institute for Organ Transplantation (GIOT) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Brain Death Tissue and Organ Procurement renal injury biomarkers Endothelium Inflammation endothelial activation TRACE DETERMINATION medicine.disease_cause Kidney Peritubular capillaries Endothelial activation Von Willebrand factor HUMAN-LIVER Internal medicine medicine Immunology and Allergy Humans oxidative stress Pharmacology (medical) DONOR KIDNEYS thrombosis SELECTIN GLYCOPROTEIN LIGAND TISSUE DISTRIBUTION Transplantation biology business.industry inflammatory response ORGAN DONORS Tissue Donors DYSFUNCTION Endothelial stem cell RAT-KIDNEY medicine.anatomical_structure Endocrinology Kidney Tubules FIBRINOGEN BIOSYNTHESIS Brain Injuries Postmortem Changes biology.protein Endothelium Vascular medicine.symptom business IMMUNOLOGICAL ACTIVATION Oxidative stress |
| Zdroj: | American Journal of Transplantation, 8(5), 933-941. Wiley |
| ISSN: | 1600-6135 |
| Popis: | Cerebral injury leading to brain death (BD) causes major physiologic derangements in potential organ donors, which may result in vascular-endothelial activation and affect posttransplant graft function. We investigated the kinetic of pro-coagulatory and pro-inflammatory endothelial activation and the subsequent oxidative stress and renal tubular injury, early after BD declaration. BD was induced by slowly inflating a balloon-catheter inserted in the extradural space over a period of 30 min. Rats (n = 30) were sacrificed 0.5, 1, 2 or 4 h after BD-induction and compared with sham-controls. This study demonstrates immediate pro-coagulatory and pro-inflammatory activation of vascular endothelium after BD in kidney donor rats, proportional with the duration of BD. E- and P-Selectins, A alpha/B beta-fibrinogen mRNA were abruptly and progressively up-regulated from 0.5 h BD onwards; P-Selectin membrane protein expression was increased; fibrinogen was primarily visualized in the peritubular capillaries. Plasma von Willebrand factor was significantly higher after 2 h and 4 h BD. Urine heart-fatty-acid-binding-protein and N-acetyl-glucosaminidase, used as new specific and sensitive markers of proximal and distal tubular damage, were found significantly increased after 0.5 h, with a maximum at 4 h. Unexpectedly, oxidative stress was detectable only late, after the installation of tubular injury, suggesting only a secondary role for hypoxia in triggering these injuries. |
| Databáze: | OpenAIRE |
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