Spermine attenuates behavioral and biochemical alterations induced by quinolinic acid in the striatum of rats
| Autor: | Gerusa Duarte Dalmolin, Carlos Fernando Mello, Nádia Aléssio Velloso, V.D.G. Sinhorin, Graciela Fonini, Maribel Antonello Rubin, Aron Ferreira da Silveira |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Agonist medicine.drug_class Biguanides Spermine Striatum Motor Activity Pharmacology Biology Binding Competitive Receptors N-Methyl-D-Aspartate Antioxidants chemistry.chemical_compound medicine Animals Rats Wistar Molecular Biology Brain Chemistry Binding Sites Behavior Animal Dose-Response Relationship Drug General Neuroscience Glutamate receptor Polyamine binding Corpus Striatum Rats Quinolinic Acids Oxidative Stress chemistry Biochemistry NMDA receptor Neurology (clinical) Polyamine Excitatory Amino Acid Antagonists Developmental Biology Quinolinic acid |
| Zdroj: | Brain Research. 1198:107-114 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2007.12.056 |
| Popis: | Polyamines are aliphatic amines containing nucleophilic centers that are found in all eukaryotic cells, including brain cells. These compounds determine neuroprotection in experimental models of cerebral ischemia and neurotoxicity. In the current study we investigated the protective effects of spermine, an agonist of the polyamine binding site at the N-methyl-d-aspartate receptor, against the behavioral and neurochemical alterations induced by quinolinic acid. The unilateral intrastriatal injection of quinolinic acid (180 nmol/site into the dorsal striatum) induced stereotypical motor asymmetries, assessed by the open field and elevated body swing tests. Spermine modulated quinolinic acid-induced rotational behavior biphasically. While the previous intrastriatal administration of spermine at the dose of 0.1 nmol/site increased, the administration of spermine at the dose of 10 nmol/site reduced quinolinic acid-induced rotational behavior. Spermine (10 nmol/site) also decreased the contralateral swing behavior induced by quinolinic acid. Furthermore, the effect of 10 nmol of spermine was counteracted by the co-administration of arcaine (10 nmol), a selective antagonist of the polyamine binding site at the N-methyl-d-aspartate receptor. In addition, spermine (10 nmol) protected against quinolinic acid-induced protein carbonylation in the rat striatum, further suggesting an antioxidant role for this polyamine. These results provide evidence that the behavioral and biochemical alterations induced by quinolinic acid are attenuated or prevented by spermine through its interaction with N-methyl-d-aspartate receptor and/or its antioxidant function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect