Hippocampal neural progenitor cells play a distinct role in fear memory retrieval in male and female CIE rats
| Autor: | Khush M. Kharidia, Britta D. Sibley, Chitra D. Mandyam, Karthik K. Mysore, Michael J. Terranova, Jefferson Williams, McKenzie J. Fannon, Dvijen C. Purohit, Miranda C. Staples, Sucharita S. Somkuwar, Leon W. Quach, Janna S. Sage-Sepulveda, Roberto J. Morales Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Receptor expression Conditioning Classical Synaptophysin Hippocampus Context (language use) Hippocampal formation Biology Article 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neural Stem Cells Memory Internal medicine Glial Fibrillary Acidic Protein medicine Basic Helix-Loop-Helix Transcription Factors Animals Humans Rats Long-Evans Fear conditioning Pharmacology Sex Characteristics Ethanol Dentate gyrus Central Nervous System Depressants Fear Synaptoporin Alcoholism 030104 developmental biology Endocrinology Ki-67 Antigen Memory consolidation Female Rats Transgenic 030217 neurology & neurosurgery |
| Popis: | Adult male and female GFAP-TK transgenic rats experienced six weeks of chronic intermittent ethanol vapor inhalation (CIE). During the last week of CIE, a subset of male and female TK rats were fed with Valcyte to ablate neural progenitor cells (NPCs). Seventy-two hours after CIE cessation, all CIE and age-matched ethanol naive controls experienced auditory trace fear conditioning (TFC). Twenty-four hours later all animals were tested for cue-mediated retrieval in the fear context. Adult male CIE rats showed a significant burst in NPCs paralleled by reduction in fear retrieval compared to naive controls and Valcyte treated CIE rats. Adult female CIE rats did not show a burst in NPCs and showed similar fear retrieval compared to naive controls and Valcyte treated CIE rats, indicating that CIE-mediated impairment in fear memory and its regulation by NPCs was sex dependent. Valcyte significantly reduced Ki-67 and NeuroD labeled cells in the dentate gyrus (DG) in both sexes, demonstrating a role for NPCs in reduced fear retrieval in males. Valcyte prevented adaptations in GluN2A receptor expression and synaptoporin density in the DG in males, indicating that NPCs contributed to alterations in plasticity-related proteins and mossy fiber projections that were associated with reduced fear retrieval. These data suggest that DG NPCs born during withdrawal and early abstinence from CIE are aberrant, and could play a role in weakening long-term memory consolidation dependent on the hippocampus. |
| Databáze: | OpenAIRE |
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