Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq
| Autor: | Steffen Porwollik, Pui Cheng, Mark Rupert, Timothy G. Hammond, Phillip Stafford, J. Devich, L. Quick, R. Bober, Jörg Vogel, Patricia L. Allen, Michael McClelland, D. Fernandez, George Tsaprailis, Andrea M. Hunt, Randall W. Nelson, James McCracken, A. Ruggles, Lokesh Joshi, L. Catella, Emily G. Richter, S. Hing, C. A. Nickerson, P. Dumars, Timothy R. Radabaugh, Kent L. Buchanan, C. M. Ott, Carla Goulart, Rajee Ramamurthy, Heidemarie M. Stefanyshyn-Piper, Louis S. Stodieck, James W. Wilson, K. Höner zu Bentrup, Duane L. Pierson, Miti Shah, C. Baker-Coleman, Michael J. Schurr, Lisa A. Morici, Macarena Parra, Kelly L.L. Norwood, Michelle Kilcoyne, Mayra Nelman-Gonzalez |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Salmonella typhimurium Iron Regulator Virulence Gene Expression Biology Host Factor 1 Protein Regulon Microbiology Mice Gene expression Animals Pathogen Weightlessness Simulation Oligonucleotide Array Sequence Analysis Genetics Mice Inbred BALB C Salmonella Infections Animal Multidisciplinary Biofilm Space Flight Biological Sciences Phenotype Genes Bacterial Biofilms Female |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 104(41) |
| ISSN: | 0027-8424 |
| Popis: | A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and logistical hurdles. Moreover, the effects of space flight on microbial pathogenicity and associated infectious disease risks have not been studied. The bacterial pathogen Salmonella typhimurium was grown aboard Space Shuttle mission STS-115 and compared with identical ground control cultures. Global microarray and proteomic analyses revealed that 167 transcripts and 73 proteins changed expression with the conserved RNA-binding protein Hfq identified as a likely global regulator involved in the response to this environment. Hfq involvement was confirmed with a ground-based microgravity culture model. Space flight samples exhibited enhanced virulence in a murine infection model and extracellular matrix accumulation consistent with a biofilm. Strategies to target Hfq and related regulators could potentially decrease infectious disease risks during space flight missions and provide novel therapeutic options on Earth. |
| Databáze: | OpenAIRE |
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