Turbulent fluid flow is a novel closed-system sample extraction method for flexible endoscope channels of various inner diameters
| Autor: | Michelle J. Alfa, Mohamed E. Labib, Seo Yean Sohn, Richard Lai, Yacoob Tabani |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Endoscope Extraction ratio Colony Count Microbial Positive control Microbiology Enterococcus faecalis Article 03 medical and health sciences Candida albicans Sample extraction Molecular Biology 030304 developmental biology Endoscopes 0303 health sciences Chromatography biology 030306 microbiology Chemistry Extraction (chemistry) Biofilm biology.organism_classification Disinfection Biofilms Pseudomonas aeruginosa Hydrodynamics Flexible endoscope Equipment Contamination Shear Strength |
| Zdroj: | J Microbiol Methods |
| ISSN: | 1872-8359 |
| Popis: | Overview Effective sample extraction from endoscope channels is crucial for monitoring manual cleaning adequacy as well as for ensuring optimal sensitivity for culture after disinfection. The objective of this study was to compare the efficacy of Turbulent Fluid Flow (TFF) to Flush (F) or Flush-Brush-Flush (FBF) methods. Materials & methods Pseudomonas aeruginosa and Enterococcus faecalis in artificial test soil-2015 (ATS2015) were used as bacterial markers while protein and carbohydrate were the organic markers for biofilm formed inside 3.2-mm and 1.37-mm polytetrafluoroethylene (PTFE) channels. TFF was generated using compressed air and sterile water to provide friction for sample extraction. Extraction for biofilm coated PTFE channels as well as for colonoscope channels perfused with ATS2015 containing 108 CFU/mL P. aeruginosa, E. faecalis and Candida albicans was determined using TFF compared to FBF and F. Results The extraction ratio for P. aeruginosa and E. faecalis from biofilm extracted by TFF compared to the positive control was significantly better than F for 1.37-mm channels (≥0.94 for both bacteria by TFF versus 0.69 to 0.72 by F for P. aeruginosa and E. faecalis, respectively) but not significantly different between TFF and FBF for 3.2-mm channels. F was also ineffective for extraction of protein and carbohydrate from 1.37-mm channels. Extraction efficacy by TFF from inoculated colonoscope channels was >98% for all test markers. Conclusions The novel TFF method for extraction of samples from colonoscope channels is a more effective method than the existing FBF and F methods. |
| Databáze: | OpenAIRE |
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