Production of monoclonal antibodies against the purified glycosylphosphatidylinositol anchor of the variant surface glycoprotein from Trypanosoma brucei brucei
| Autor: | Johannes Gunawan, Frank Schuler, Jasna Peter-Katalinic, Heinz Egge, Ute Preuβ |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Immunodiffusion
Glycosylphosphatidylinositols medicine.drug_class Blotting Western Immunoblotting Molecular Sequence Data Trypanosoma brucei brucei Biophysics Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Spectrometry Mass Fast Atom Bombardment Trypanosoma brucei Phosphatidylinositols Monoclonal antibody Immunofluorescence Biochemistry Mass Spectrometry Mice Western blot medicine Animals Molecular Biology chemistry.chemical_classification Mice Inbred BALB C biology medicine.diagnostic_test Antibodies Monoclonal Ouchterlony double immunodiffusion biology.organism_classification Molecular biology Carbohydrate Sequence chemistry Polyclonal antibodies biology.protein Glycolipids Glycoprotein Variant Surface Glycoproteins Trypanosoma |
| Zdroj: | Archives of Biochemistry and Biophysics. 291:139-146 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(91)90116-z |
| Popis: | The glycosylphosphatidylinositol anchor (GPI) from the membrane form variant surface glycoprotein (mfVSG) of Trypanosoma brucei brucei was isolated and identified after radioactive labeling with [3H]myristic acid, by immunostaining on HPTLC with a polyclonal antibody directed against mfVSG and by negative ion laser desorption and fast atom bombardment mass spectrometry of the GPI anchor before and after peracetylation. For the production of monoclonal antibodies the purified GPI molecule was incorporated into liposomes and injected intrasplenically in BALB/c mice. After fusion with the myeloma cell line X63-Ag 8.653 hybridoma cells were cloned by single cell cloning. The secreted antibodies were characterized by ELISA, Ouchterlony immunodiffusion, and Western blot and used in first immunofluorescent studies. |
| Databáze: | OpenAIRE |
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