Calretinin immunoreactivity in pineal gland of different mammals including man
| Autor: | Radivoj V. Krstić, Dominique Nicolas, Alain Novier |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Pathology medicine.medical_specialty Blotting Western Immunocytochemistry Nerve Tissue Proteins Ovary Biology Pineal Gland Pinealocyte Immunoenzyme Techniques Guinea pig Pineal gland S100 Calcium Binding Protein G Endocrinology Parenchyma medicine Animals Humans Rats Wistar Fluorescent Antibody Technique Indirect Microscopy Immunoelectron Aged Aged 80 and over Goats Middle Aged Rats medicine.anatomical_structure Calbindin 2 Cattle Female Calretinin Gerbillinae Endocrine gland |
| Zdroj: | Journal of Pineal Research. 21:121-130 |
| ISSN: | 1600-079X 0742-3098 |
| DOI: | 10.1111/j.1600-079x.1996.tb00279.x |
| Popis: | Calretinin (CR) is a calcium-binding protein, found in a variety of organs and systems such as the central nervous system and the pineal gland. It was first thought to be a specific neuronal marker but this selectivity is now in question since CR has been demonstrated in avian thymus, rat ovary, rat and guinea pig inner ear, rat testis, and chicken and rat pineal gland. To contribute to the knowledge of the presence of CR-positive cells in the pineal parenchyma of rat and other mammalian including man, we performed immunocytochemistry on pineal glands of gerbils, rats, goats, cows, and humans, using a CR anti-serum. To confirm it was actually CR that was demonstrated, we performed Western Blot analyses. Finally, to precisely identify the nature of CR-positive cells we accomplished double-labelling immunofluorescence, using antisera against some nerve cell specific cytosquelettal proteins such as MAP-5, MAP-2, NF-L, NF-M, and NF-H. CR-positive cells were found in all pineal glands studied. These cells all possess a round, oval, or polygonal-shaped perikaryon sending one or more processes of different lengths into the glandular parenchyma. There is a lack of CR immunoreactivity in the nucleus and cell organelles while the cytosol contains a high concentration of this protein. Nevertheless, there are some slight differences between species, especially concerning the number of reactive cells and their relationships with different parenchymal structures such as blood vessels or acervuli. Among the CR-positive cells, only a few were actually nerve cells, contributing probably to an intrinsic innervation of the gland. The remaining CR-reactive cells seem to correspond mostly to pinealocytes in a specific histophysiological state and possibly to neuron-like cells. The significance of the CR-positive cells in the pineal glands remains to be elucidated. |
| Databáze: | OpenAIRE |
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