Determination of VEGFR-2 (KDR) 604A>G Polymorphism in Pancreatic Disorders
| Autor: | Dragoş Ovidiu Alexandru, Mircea Cătălin ForŢofoiu, Marius Eugen Ciurea, Mihail Virgil Boldeanu, Mihai Ioana, Valeriu Surlin, Mihai Gabriel Cucu, Elena Mădălina Dumitrescu, Lidia Boldeanu, Maria ForŢofoiu, Isabela Silosi, F Petrescu, Ioana Streaţă, Vlad Pădureanu, Anca Ştefania Enescu, Adrian Saftoiu, Maria Bogdan, Aurelia Enescu, Ileana Octavia Petrescu |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty genotype Single-nucleotide polymorphism Bioinformatics Polymorphism Single Nucleotide Gastroenterology Article Catalysis polymorphism lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Internal medicine Pancreatic cancer Genotype medicine Humans SNP Physical and Theoretical Chemistry Allele lcsh:QH301-705.5 Molecular Biology Spectroscopy Demography business.industry Organic Chemistry pancreatic disorders Pancreatic Diseases Kinase insert domain receptor General Medicine Middle Aged medicine.disease Vascular Endothelial Growth Factor Receptor-2 VEGFR-2 Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 Case-Control Studies 030220 oncology & carcinogenesis Pancreatitis Acute pancreatitis Female 030211 gastroenterology & hepatology business |
| Zdroj: | International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 439 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 18, Iss 2, p 439 (2017) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms18020439 |
| Popis: | Pancreatic disorders have a high prevalence worldwide. Despite the fact that screening methods became more effective and the knowledge we have nowadays about pancreatic diseases has enhanced, their incidence remains high. Our purpose was to determine whether single nucleotide polymorphism (SNP) of VEGFR-2/KDR (vascular endothelial growth factor receptor 2/kinase insert domain receptor) influences susceptibility to develop pancreatic pathology. Genomic DNA was extracted from blood samples collected from patients diagnosed with acute pancreatitis (n = 110), chronic pancreatitis (n = 25), pancreatic cancer (n = 82) and healthy controls (n = 232). VEGFR-2 (KDR) 604A>G (rs2071559) polymorphism frequency was determined with TaqMan allelic discrimination assays. Statistical assessment was performed by associating genetic polymorphism with clinical and pathological data. In both pancreatic disorders and healthy control groups the polymorphism we studied was in Hardy-Weinberg equilibrium. Association between increased risk for pancreatic disorders and studied polymorphism was statistically significant. KDR 604AG and AG + GG genotypes were more prevalent in acute pancreatitis and pancreatic cancer patients than in controls. These genotypes influence disease development in a low rate. No association was found between chronic pancreatitis and KDR 604AG and AG + GG genotypes. In Romanian cohort, we found an association between the KDR 604A→G polymorphism and acute pancreatitis and pancreatic cancer. Carriers of the -604G variant allele were more frequent among acute pancreatitis and pancreatic cancer than among controls, suggesting that KDR 604G allele may confer an increased risk for these diseases. In the future, more extensive studies on larger groups are necessary, in order to clarify the role of VEGFR2 polymorphisms in pancreatic pathology. |
| Databáze: | OpenAIRE |
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