1,25-Dihydroxyvitamin D3 enhances neural stem cell proliferation and oligodendrocyte differentiation

Autor: Bogoljub Ciric, Guang Xian Zhang, Hasti Atashi Shirazi, Abdolmohamad Rostami, Javad Rasouli
Rok vydání: 2015
Předmět:
medicine.medical_specialty
Encephalomyelitis
Autoimmune
Experimental
Multiple Sclerosis
Cellular differentiation
Clinical Biochemistry
Ciliary neurotrophic factor
Article
Pathology and Forensic Medicine
Mice
Calcitriol
Neural Stem Cells
Neurotrophin 3
Neurotrophic factors
Internal medicine
medicine
Glial cell line-derived neurotrophic factor
Animals
Ciliary Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
Molecular Biology
Cells
Cultured
Cell Proliferation
Neurons
biology
Brain-Derived Neurotrophic Factor
Experimental autoimmune encephalomyelitis
Oligodendrocyte differentiation
Cell Differentiation
medicine.disease
Oligodendrocyte
Neural stem cell
Cell biology
Up-Regulation
Mice
Inbred C57BL
Disease Models
Animal
Oligodendroglia
medicine.anatomical_structure
Endocrinology
Neuroprotective Agents
nervous system
Astrocytes
biology.protein
Receptors
Calcitriol
Female
Zdroj: Experimental and molecular pathology. 98(2)
ISSN: 1096-0945
Popis: 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has recently been found to suppress experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Although its effect was attributed to an anti-inflammatory mechanism, it is not clear whether this treatment can also directly act on neural cells to promote CNS recovery. The present study investigates the effect of various concentrations of 1,25(OH)2D3 on neural stem cell (NSC) proliferation and their differentiation to oligodendrocytes, the myelinating cells. We have, for the first time, shown that NSCs constitutively express vitamin D receptor (VDR), which can be upregulated by 1,25(OH)2D3. This vitamin significantly enhanced proliferation of NSCs, and enhanced their differentiation into neurons and oligodendrocytes, but not astrocytes. NSCs treated with 1,25(OH)2D3 showed increased expression of NT-3, BDNF, GDNF and CNTF, important neurotrophic factors for neural cell survival and differentiation. Overall, we demonstrated that 1,25(OH)2D3 has a direct effect on NSC proliferation, survival, and neuron/oligodendrocyte differentiation, thus representing a novel mechanism underlying its remyelinating and neuroprotective effect in MS/EAE therapy.
Databáze: OpenAIRE
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