Protective effect of diet supplemented with rice prolamin extract against DNCB-induced atopic dermatitis in BALB/c mice
Autor: | Kwang Il Nam, Chang-Sub Ku, Bong-Whan Ahn, Seong Jin Kim, Hyun-Woo Kim, Choon-Sang Bae, Dong-Il Jang, Mi-Sun Jang, Dong-Up Song, Hyun-Joong Yoon, Seung-Rock Lee |
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Rok vydání: | 2015 |
Předmět: |
medicine.medical_specialty
Anti-Inflammatory Agents Immunoglobulin E BALB/c law.invention Dermatitis Atopic Mice Interferon-gamma Dermis Downregulation and upregulation law Internal medicine medicine Animals Interferon gamma Skin Atopic dermatitis Transepidermal water loss Mice Inbred BALB C biology business.industry Plant Extracts Oryza General Medicine Dietary rice prolamin extract biology.organism_classification medicine.disease Disease Models Animal medicine.anatomical_structure Endocrinology Complementary and alternative medicine Immunology biology.protein Female sense organs business Phytotherapy medicine.drug Prolamins Research Article |
Zdroj: | BMC Complementary and Alternative Medicine BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(15) |
ISSN: | 1472-6882 |
Popis: | Background: Rice prolamin has been reported to possess antioxidative, anti-inflammatory and immune-promoting properties. This study is aimed to examine the protective effects of dietary rice prolamin extract (RPE) against dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in mice. Methods: BALB/c mice were fed diet supplemented with 0-0.1 % RPE for 6 weeks. For the last 2 weeks, 1 % or 0.2 % DNCB was applied repeatedly to the back skin of mice to induce AD-like lesions. Following AD induction, the severity of skin lesions was examined macroscopically and histologically. In addition, the serum levels of IgE, IgG1 and IgG2a were determined by ELISA, and the mRNA expression of IL-4 and IFN-gamma in the skin was determined by real-time PCR. Results: Dietary RPE suppressed the clinical symptoms of DNCB-induced dermatitis as well as its associated histopathological changes such as epidermal hyperplasia and infiltration of mast cells and eosinophils in the dermis. RPE treatment also suppressed the DNCB-induced increase in transepidermal water loss. Dietary RPE inhibited the DNCB-induced enhancement of serum IgE and IgG1 levels, whereas it increased the serum IgG2a level in DNCB-treated mice. In addition, dietary RPE upregulated the IFN-gamma mRNA expression and downregulated the IL-4 mRNA expression in the skin of DNCB-treated mice. Conclusions: The above results suggest that dietary RPE exerts a protective effect against DNCB-induced AD in mice via upregulation of Th1 immunity and that RPE may be useful for the treatment of AD. |
Databáze: | OpenAIRE |
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