Multiplication of Ribosomal P-Stalk Proteins Contributes to the Fidelity of Translation
| Autor: | Monika Szajwaj, Barbara Michalec-Wawiórka, Leszek Wawiórka, Lidia Borkiewicz, Mateusz Molon, Eliza Molestak, Aleksandra Boguszewska, Marek Tchórzewski, Przemysław Grela |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ribosomal Proteins Proteome Cell GTPase Saccharomyces cerevisiae Biology Ribosome GTP Phosphohydrolases 03 medical and health sciences Ribosomal protein medicine Protein biosynthesis Molecular Biology Translation (biology) Cell Biology Ribosomal RNA Phosphoproteins Cell biology Protein Structure Tertiary 030104 developmental biology medicine.anatomical_structure Polyribosomes Ribosomes Research Article |
| Zdroj: | Molecular and cellular biology. 37(17) |
| ISSN: | 1098-5549 |
| Popis: | The P-stalk represents a vital element within the ribosomal GTPase-associated center, which represents a landing platform for translational GTPases. The eukaryotic P-stalk exists as a uL10-(P1-P2)2 pentameric complex, which contains five identical C-terminal domains, one within each protein, and the presence of only one such element is sufficient to stimulate factor-dependent GTP hydrolysis in vitro and to sustain cell viability. The functional contribution of the P-stalk to the performance of the translational machinery in vivo, especially the role of P-protein multiplication, has never been explored. Here, we show that ribosomes depleted of P1/P2 proteins exhibit reduced translation fidelity at elongation and termination steps. The elevated rate of the decoding error is inversely correlated with the number of the P-proteins present on the ribosome. Unexpectedly, the lack of P1/P2 has little effect in vivo on the efficiency of other translational GTPase (trGTPase)-dependent steps of protein synthesis, including translocation. We have shown that loss of accuracy of decoding caused by P1/P2 depletion is the major cause of translation slowdown, which in turn affects the metabolic fitness of the yeast cell. We postulate that the multiplication of P-proteins is functionally coupled with the qualitative aspect of ribosome action, i.e., the recoding phenomenon shaping the cellular proteome. |
| Databáze: | OpenAIRE |
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