Cytotoxicity and mitogenicity assays with real-time and label-free monitoring of human granulosa cells with an impedance-based signal processing technology intergrating micro-electronics and cell biology
| Autor: | Filiz Senbabaoglu, Bulent Urman, Nazli Akin, Ozgur Oktem, Defne Urman, Gamze Bildik, Akira Iwase, Yilmaz Guzel, Nathan A. Lack, Feridun Yakar, Basak Balaban |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival DNA damage Population Cell Antineoplastic Agents Apoptosis Biology Toxicology 03 medical and health sciences Toxicity Tests Electric Impedance medicine Humans Cytotoxic T cell Viability assay education Cytotoxicity Cells Cultured Progesterone Cell Proliferation Cisplatin education.field_of_study Granulosa Cells Estradiol Cell growth Signal Processing Computer-Assisted Cell biology 030104 developmental biology medicine.anatomical_structure Biological Assay Female Mitogens DNA Damage medicine.drug |
| Zdroj: | Reproductive Toxicology. 60:82-91 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2015.12.005 |
| Popis: | A recently developed technology (xCelligence) integrating micro-electronics and cell biology allows real-time, uninterrupted and quantitative analysis of cell proliferation, viability and cytotoxicity by measuring the electrical impedance of the cell population in the wells without using any labeling agent. In this study we investigated if this system is a suitable model to analyze the effects of mitogenic (FSH) and cytotoxic (chemotherapy) agents with different toxicity profiles on human granulosa cells in comparison to conventional methods of assessing cell viability, DNA damage, apoptosis and steroidogenesis. The system generated the real-time growth curves of the cells, and determined their doubling times, mean cell indices and generated dose-response curves after exposure to cytotoxic and mitogenic stimuli. It accurately predicted the gonadotoxicity of the drugs and distinguished less toxic agents (5-FU and paclitaxel) from more toxic ones (cisplatin and cyclophosphamide). This platform can be a useful tool for specific end-point assays in reproductive toxicology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect