Peroxisomal ABC transporters and beta-oxidation during the life cycle of the filamentous fungus Podospora anserina
| Autor: | Véronique Berteaux-Lecellier, Denise Zickler, Eric Espagne, Anne Bourdais, Anne-Laure Riquet, Stéphanie Boisnard |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Oxidation-Reduction
MESH: Mitochondria Genes Fungal ATP-binding cassette transporter Fatty acid degradation Microbiology Podospora anserina Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Podospora MESH: Spores Fungal Gene Expression Regulation Fungal MESH: Podospora Genetics Peroxisomes [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Beta oxidation Peroxisomal targeting signal 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030302 biochemistry & molecular biology Fatty Acids MESH: Oleic Acid Fatty acid Peroxisome Spores Fungal biology.organism_classification MESH: Peroxisomes Mitochondria MESH: Fatty Acids [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry MESH: ATP-Binding Cassette Transporters ATP-Binding Cassette Transporters MESH: Fungal Proteins MESH: Genes Fungal Oxidation-Reduction MESH: Gene Expression Regulation Fungal Oleic Acid |
| Zdroj: | Fungal Genetics and Biology Fungal Genetics and Biology, Elsevier, 2009, 46 (1), pp.55-66. ⟨10.1016/j.fgb.2008.10.006⟩ |
| ISSN: | 1087-1845 1096-0937 |
| DOI: | 10.1016/j.fgb.2008.10.006⟩ |
| Popis: | International audience; ATP-binding cassette transporters are ubiquitous proteins that facilitate transport of diverse substances across a membrane. However, their exact role remains poorly understood. In order to test their function in a fungus life cycle, we deleted the two Podospora anserina peroxisomal ABC transporter pABC1 and pABC2 genes as well as the three genes involved in peroxisomal (fox2) and mitochondrial (scdA and echA) beta-oxidation. Analysis of the single and double mutants shows that fatty acid beta-oxidation occurs in both organelles. Furthermore, the peroxisomal and mitochondrial fatty acid beta-oxidation pathways are both dispensable for vegetative and sexual development. They are, however, differently required for ascospore pigmentation and germination, this latter defect being restored in a DeltapABC1 and DeltapABC2 background. We report also that lack of peroxisomal ABC transporters does not prevent peroxisomal long-chain fatty acid oxidation, suggesting the existence of another pathway for their import into peroxisomes. Finally, we show that some aspects of fatty acid degradation are clearly fungus species specific. |
| Databáze: | OpenAIRE |
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