Neural-Competent Cells of Adult Human Dermis Belong to the Schwann Lineage
| Autor: | David Otaegui, Ander Matheu, Haizea Iribar, Izaskun Burgoa, Virginia Pérez-López, Mario García-Domínguez, Maria Paz Lopez-Mato, Araika Gutiérrez-Rivera, Olatz Leis, Ariane Aduriz, Angel G. Martin, Ander Izeta, Haritz Irizar, Maider Muñoz-Culla, Nuria Gago-Lopez, Adrián Pérez-San Vicente, Robb MacLellan, Usue Etxaniz, Ainara Vallejo-Illarramendi |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, Eusko Jaurlaritza, European Commission |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Lineage (genetic) Adolescent Cell Foreskin Nerve Tissue Proteins Receptors Nerve Growth Factor Biology Biochemistry Article Transcriptome Mice Young Adult SOX2 Dermis Neural Stem Cells Precursor cell Genetics medicine Animals Humans Cell Lineage Child lcsh:QH301-705.5 Cells Cultured Aged Neurons lcsh:R5-920 Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Infant Cell Differentiation Cell Biology Middle Aged CD56 Antigen Cell biology stomatognathic diseases medicine.anatomical_structure lcsh:Biology (General) Child Preschool Immunology Neural differentiation Schwann Cells lcsh:Medicine (General) Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Stem Cell Reports Stem Cell Reports, Vol 3, Iss 5, Pp 774-788 (2014) |
| ISSN: | 2213-6711 |
| DOI: | 10.1016/j.stemcr.2014.09.009 |
| Popis: | Summary Resident neural precursor cells (NPCs) have been reported for a number of adult tissues. Understanding their physiological function or, alternatively, their activation after tissue damage or in vitro manipulation remains an unsolved issue. Here, we investigated the source of human dermal NPCs in adult tissue. By following an unbiased, comprehensive approach employing cell-surface marker screening, cell separation, transcriptomic characterization, and in vivo fate analyses, we found that p75NTR+ precursors of human foreskin can be ascribed to the Schwann (CD56+) and perivascular (CD56−) cell lineages. Moreover, neural differentiation potential was restricted to the p75NTR+CD56+ Schwann cells and mediated by SOX2 expression levels. Double-positive NPCs were similarly obtained from human cardiospheres, indicating that this phenomenon might be widespread. Graphical Abstract Highlights • Human dermis-derived cultures show two types of SOX2+ cells: Schwann and perivascular • p75NTR+CD56+ Schwann cells are responsible for neural progeny • SOX2 expression levels regulate the neural competence of dermal precursors • p75NTR+CD56+ neural precursor cells similarly arise from human cardiospheres In this article, Izeta and colleagues show that neural precursor cell (NPC) cultures from adult human dermis include two SOX2+ cell subpopulations (Schwann and perivascular), and neural progeny is derived only from Schwann cells in a process regulated by their SOX2 expression levels. NPCs similarly arise from human cardiospheres, potentially elucidating the origin of seemingly unrelated NPCs observed in multiple organs. |
| Databáze: | OpenAIRE |
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