Meloydogine incognita (Nematoda) reproduction affected by plants overexpressing a steroid inducible gene-expression system
| Autor: | Roberta Pastorelli, Patrizia Bogani, Tiziana Irdani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine M. incognita Nematode reproduction Plant development Progesterone Tobacco Genetics Plant Science Biology 01 natural sciences 03 medical and health sciences Transcription (biology) Auxin Botany medicine Abiotic component chemistry.chemical_classification fungi food and beverages Phenotype Cell biology 030104 developmental biology chemistry Gibberellin Terra incognita Glucocorticoid 010606 plant biology & botany Hormone medicine.drug |
| Popis: | This study reports the impact of an integral glucocorticoid expression system (GR-system) on plant and plant-nematode reproduction. Seedlings expressing a GR-system (transGR+) show affections of growth and development. It is well-known that plant-hormones such as gibberellins, auxins and steroids are essential for developmental and physiological processes, in biotic/abiotic stresses. Here is shown some phenotypic affections reported by tobacco expressing a whole mammalian GR-system, how those effects are rescued by external hormonal treatments and the influence on plant-nematode fecundity. Results demonstrate that: i) GR-system is constitutively expressed in plants and its functionality proved by the activation of the reporter-gene transcription (uidA) after induction with different steroid hormones, dexamethasone (DEX) and progesterone (PRO) as well; ii) transGR + growth affected at hypocotyls and roots development result mitigated by exogenous hormones applications; iii) no differential expression in the expansin-gene TExp4 assayed; iv) Significant reductions of J2-larvae assessed in the egg-masses and soil during the generations on transformed plants. Although the molecular mechanism behind these results is still unknown, all together these results suggest a role of GR as reflected by the impact on the plant development and reduction of Meloydogine incognita (Nematoda) second-stage juveniles in egg-masses and soil. |
| Databáze: | OpenAIRE |
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