Use of chitosan as pharmaceutical excipient in ocular drug delivery systems: Sterilization and pharmacokinetics

Autor: Leonardo Lima Fuscaldi, Giselle Foureaux, Simone Odília Antunes Fernandes, Aina Liz Alves Cesar, Juçara R. Franca, Sebastião Cronemberger, Rachel Oliveira Castilho, Anderson J. Ferreira, Valbert Nascimento Cardoso, Tatiana G. Ribeiro, André Augusto Gomes Faraco
Rok vydání: 2020
Předmět:
Zdroj: Journal of Biomedical Materials Research Part B: Applied Biomaterials. 108:2227-2237
ISSN: 1552-4981
1552-4973
Popis: The use of chitosan as a pharmaceutical excipient in the ocular field is already established. Nevertheless, some aspects related to its ocular administration, such as sterilization and excipient's pharmacokinetics, remain unclear. So, in this study, we evaluated those two relevant aspects, related to chitosan administration in eye. We used chitosan-based ocular inserts (CI) as formulation model. CI were produced by solvent/casting method and sterilized by saturated steam. Sterilization was confirmed by direct inoculation of inserts in suitable microbiological growth media. Physicochemical characterization of inserts before and after sterilization was performed. Results suggested that, although steam sterilization changed the arrangement of the matrix, the heat and the humidity did not modify the structure of the main polymeric chain. Pharmacokinetics of CI radiolabeled with technetium-99m (99m Tc) was assessed by scintigraphic images and ex vivo biodistribution study, after ocular administration in male Wistar rats. Scintigraphic and images analysis and ex vivo biodistribution study showed that the insert remained mainly in the eye until 6 hr after administration and its degradation products began to migrate to the abdominal cavity after 18 hr. Together, these data represent an important step forward the manufacturing and the clinical application of CI in the ophthalmic field.
Databáze: OpenAIRE
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