A New Approach in the Management of Urothelial Tumors Using GM-CSF on Marker Lesions: An Ultrastructural and Immunohistochemical Study on the Macrophage Population in Bladder Mucosa
| Autor: | P. Stravoravdi, E. Konstandinidis, Panagiotis Gigis, K. Dimitriadis, A. Barich, P. Kirtsis, Th. Toliou, Konstantinos Natsis |
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| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Immunology Population urologic and male genital diseases Lesion Adjuvants Immunologic Virology Biomarkers Tumor medicine Humans Urothelium education Aged Carcinoma Transitional Cell education.field_of_study Bladder cancer medicine.diagnostic_test CD68 business.industry Macrophages Granulocyte-Macrophage Colony-Stimulating Factor Cell Biology Cystoscopy Middle Aged medicine.disease Immunohistochemistry female genital diseases and pregnancy complications Microscopy Electron Transitional cell carcinoma Urinary Bladder Neoplasms Chemotherapy Adjuvant medicine.symptom business |
| Zdroj: | Journal of Interferon & Cytokine Research. 19:221-225 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/107999099314144 |
| Popis: | Our purpose was to investigate a new therapeutic model, GM-CSF-targeted immunomodulation on transitional cell carcinoma (TCC) marker lesions and to evaluate the immunologic response of the bladder mucosa. Eleven patients with pTa or pT1 bladder cancer were eligible for the study. All lesions were removed by transurethral resection (TUR) except for a marker lesion. All patients received 8 weekly instillations of 300 microg of GM-CSF, after which cystoscopy with bladder biopsies +/- TUR was repeated on adjacent urothelium or tumor or both. Paraffin-embedded sections were immunohistochemically stained with CD68, which labels monocytes and macrophages. The CD68+ cell population was evaluated as 1+ to 3+. Comparable specimens were routinely processed for ultrastructural analysis. Complete response was observed in 6 patients (55%), persistent tumor occurred in 4 patients (approximately 36.4%), and 1 patient (8.6%) showed recurrence. Immunohistochemically, an at least twofold increase in the number of the CD68+ cells was observed in all responders. Submicroscopically, migration of macrophages to the surface layer occurred. Macrophages showed an extensive lysosomal system and pseudopodia. This study indicates that the prophylactic treatment of TCC with GM-CSF may induce immunomodulatory effects on macrophage activities, which could be associated with the clinical evolution of the disease. |
| Databáze: | OpenAIRE |
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