Establishment and proteomic characterization of a novel cell line, NCC-UPS2-C1, derived from a patient with undifferentiated pleomorphic sarcoma
Autor: | Marimu Sakumoto, Rieko Oyama, Akira Kawai, Fusako Kito, Tadashi Kondo, Shunichi Toki, Kumiko Shiozawa, Akihiko Yoshida |
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Rok vydání: | 2018 |
Předmět: |
Proteomics
0301 basic medicine Proteome Cell Biology Undifferentiated Pleomorphic Sarcoma 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Biomarkers Tumor medicine Humans Cell Proliferation Aged 80 and over Cell growth Gene Expression Profiling Mesenchymal stem cell Sarcoma Cell Biology General Medicine medicine.disease Primary tumor 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Cancer research Female Stem cell Developmental Biology |
Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 54:257-263 |
ISSN: | 1543-706X 1071-2690 |
Popis: | Undifferentiated pleomorphic sarcoma (UPS) is an aggressive mesenchymal malignancy requiring novel therapeutic approaches to improve clinical outcome. Patient-derived cancer cell lines are an essential tool for investigating molecular mechanisms underlying cancer initiation and development; however, there is a lack of patient-derived cell lines of UPS available for research. The objective of this study was to develop a patient-derived cell model of UPS. A cell line designated NCC-UPS2-C1 was established from the primary tumor tissue of an 84-yr-old female patient with UPS. The short tandem repeat pattern of NCC-UPS2-C1 cells was identical to that of the original tumor and distinct from that of any other cell lines deposited in public cell banks. NCC-UPS2-C1 cells were maintained as a monolayer culture for over 80 passages during 30 mo and exhibited spindle-like morphology, continuous growth, and ability for spheroid formation and invasion. Proteomic profiling using mass spectrometry and functional treemap analysis revealed that the original tumor and the derived NCC-UPS2-C1 cells had similar but distinct protein expression patterns. Our results indicate that a novel UPS cell line was successfully established and could be used to study UPS development and effects of anti-cancer drugs. However, the revealed difference between proteomes of the original tumor and NCC-UPS2-C1 cells should be further investigated to determine the appropriate applications of this cell line in UPS research. |
Databáze: | OpenAIRE |
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