Expression of 15-lipoxygenase-1 in human nasal epithelium: Its implication in mucociliary differentiation
| Autor: | Kyung-Su Kim, Jeung Gweon Lee, Joo-Heon Yoon, Joohwan Lee, Hee-Sun Chun, Jong-Bum Yoo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genetic Markers
Pathology medicine.medical_specialty Clinical Biochemistry Retinoic acid Gene Expression Tretinoin Mucous membrane of nose Biology Mice chemistry.chemical_compound Lipoxygenase Gene expression medicine Animals Arachidonate 15-Lipoxygenase Humans Cilia Postnatal day Cells Cultured chemistry.chemical_classification Mucins Cell Differentiation Cell Biology Nasal epithelium Epithelium Culture Media Up-Regulation Cell biology Mucus Nasal Mucosa Enzyme medicine.anatomical_structure chemistry biology.protein Interleukin-4 |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 73:77-83 |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/j.plefa.2005.05.023 |
| Popis: | 15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here, we investigated the relation between 15-LO-1 expression and the differentiation of human nasal epithelium. In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in normal human nasal epithelial cell time-dependently increased, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 at 1 ng/ml concentration time-dependently induced 15-LO-1 expression. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In murine nasal mucosa, the expression of leukocyte type-12-LO, a functional equivalent of 15-LO-1, reduced after postnatal day 7. Our findings suggest that 15-LO-1 is related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of human nasal epithelium. |
| Databáze: | OpenAIRE |
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