| Autor: |
Viji Shridhar, Alfonso Baldi, Jeremy Chien, Wilma Lingle, Robert J. Linhardt, Bo Yang, Kaustubh Datta, Bruno Vincenzi, Laura Lorenzon, Pasquale Mellone, Peng Liu, Ashwani Khurana |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22390554.v1 |
| Popis: |
Supplementary Materials and Methods. Figure S1: Quantitative Real-time PCR. Figure S2: MCF7 cells were transfected with HIF-1α siRNA oligos and control siRNA oligos before exposing them to hypoxia (3% oxygen) for indicated time intervals. Figure S3 : MTT assay was performed on MCF10DCIS batch clones after 24 hours of treatments as indicated. Figure S4: (A and B) pcDNA3.1 or pcDNA3.1 HSulf-1-myc/His plasmid transfected MCF10DCIS. Figure S5: K means cluster analysis of HSulf-1 expression with hypoxia signature genes as described in supplementary materials and methods. Figure S6: Kaplan-Meier survival analysis was performed to evaluate HSulf-1 mRNA expression levels with disease-free and overall patient survival. Figure S7: Proposed model. Table S1: Primers used for cloning, ChIP and siRNA oligos. Table S2: Patient Characteristics. Table S3: Chi-square statistical analysis of HSulf-1 and CAIX expression. Table S4: Prognostic factors in breast cancer cases compared with disease-free survival. Table S5: Prognostic factors in breast cancer cases compared with overall survival. Table S6: Characteristics of clinical parameters of breast cancer and HSulf-1 mRNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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