The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis

Autor: Zhang Qian, María D. Martín-Bermudo, Alexis Leah Braun, Morgane Guermont, David M. Glover, Francesco Meghini, Elisa Fernandez-Martinez, Yuu Kimata, Acaimo González-Reyes, Gema Villa-Fombuena
Přispěvatelé: Braun, Alexis Leah [0000-0001-6298-4832], González-Reyes, Acaimo [0000-0003-3048-0968], Kimata, Yuu [0000-0001-6223-1320], Apollo - University of Cambridge Repository, Cancer Research UK, ShanghaiTech University, Wellcome Trust Sanger Institute
Rok vydání: 2021
Předmět:
Centrosomes
germline
APC/C
Fertility
Oogenesis
0302 clinical medicine
Drosophila Proteins
Polo kinase
APC/C
Biology (General)
centrosomes
Sequence Deletion
fertility
0303 health sciences
Protein Stability
General Neuroscience
Cell biology
medicine.anatomical_structure
Ubiquitin ligase complex
Drosophila
Drosophila
germline
Genotype
QH301-705.5
Immunology
Protein Serine-Threonine Kinases
Biology
PLK1
Anaphase-Promoting Complex-Cyclosome
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Research articles
medicine
Animals
Alleles
030304 developmental biology
Microtubule nucleation
Centrosome
Base Sequence
Intercellular transport
Research
Biological Transport
Oocyte
Gene Expression Regulation
Proteolysis
Ubiquitin-Conjugating Enzymes
Oocytes
Anaphase-promoting complex
Biomarkers
030217 neurology & neurosurgery
Zdroj: Open Biology, Vol 11, Iss 6 (2021)
Digital.CSIC. Repositorio Institucional del CSIC
instname
DOI: 10.17863/cam.72059
Popis: © 2021 The Authors..
A feature of metazoan reproduction is the elimination of maternal centrosomes from the oocyte. In animals that form syncytial cysts during oogenesis, including Drosophila and human, all centrosomes within the cyst migrate to the oocyte where they are subsequently degenerated. The importance and the underlying mechanism of this event remain unclear. Here, we show that, during early Drosophila oogenesis, control of the Anaphase Promoting Complex/Cyclosome (APC/C), the ubiquitin ligase complex essential for cell cycle control, ensures proper transport of centrosomes into the oocyte through the regulation of Polo/Plk1 kinase, a critical regulator of the integrity and activity of the centrosome. We show that novel mutations in the APC/C-specific E2, Vihar/Ube2c, that affect its inhibitory regulation on APC/C cause precocious Polo degradation and impedes centrosome transport, through destabilization of centrosomes. The failure of centrosome migration correlates with weakened microtubule polarization in the cyst and allows ectopic microtubule nucleation in nurse cells, leading to the loss of oocyte identity. These results suggest a role for centrosome migration in oocyte fate maintenance through the concentration and confinement of microtubule nucleation activity into the oocyte. Considering the conserved roles of APC/C and Polo throughout the animal kingdom, our findings may be translated into other animals.
This work was supported by the Cancer Research UK Career Development Fellowship (CRUK-A12874) and the ShanghaiTech University start-up grant (2018F0202-000-06) to Y.K. and the Wellcome Trust Institutional Strategic Support Fund (ISSF) to A.L.B. and Cancer Research UK (CRUK-RG78567).
Databáze: OpenAIRE
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