National surveillance pilot study unveils a multicenter, clonal outbreak of VIM-2-producing Pseudomonas aeruginosa ST111 in the Netherlands between 2015 and 2017

Autor: I.T.M.A. Overdevest, A. Voss, Marga G van Santen-Verheuvel, M. den Reijer, J. H. Oudbier, M. van der Vusse, S. Dinant, A.G.M. Buiting, A. Stam, B.M.W. Diederen, T. Halaby, P. Schneeberger, B. Zwart, A.E. Muller, Sabine C. de Greeff, M.L. van Ogtrop, E.P.M. van Elzakker, R. Steingrover, A.L.M. Vlek, Heiman F. L. Wertheim, Sandra Witteveen, A. Troelstra, Daan W. Notermans, Jan Kluytmans, D. C. Melles, E. de Jong, E. Heikens, J.W. Dorigo-Zetsma, E.E. Mattsson, Jannette Pirzadian, S. Paltansing, W. Silvis, M. Damen, M. van der Linden, I. J. B. Spijkerman, J. C. Sinnige, T. Schulin, A. Maijer-Reuwer, M. van Rijn, Marjolein C. Persoon, S.P. van Mens, P. de Man, A.J. van Griethuysen, R.W. Bosboom, Erik Bathoorn, J.W. Cohen Stuart, Leo M. Schouls, H.M.E. Frénay, Cornelia C. H. Wielders, K.R.A. van Dijk, D.W. van Dam, Annelot F. Schoffelen, Juliëtte A. Severin, T. A. M. Trienekens, E. I. G. B. de Brauwer, K. Waar, Margreet C. Vos, M.A. Leversteijn-van Hall, A. van Dam, Corné H. W. Klaassen, M. van Trijp, M.J.H.M. Wolfhagen, Marcel G. Mennen, L. Bode, N. Roescher, A. Ott, F.S. Stals
Přispěvatelé: Medical Microbiology and Infection Prevention, Nursing, APH - Aging & Later Life, APH - Health Behaviors & Chronic Diseases, AII - Infectious diseases, Experimental Immunology, Medical Microbiology & Infectious Diseases, Anatomy and neurosciences, AMS - Ageing & Vitality, AMS - Tissue Function & Regeneration, Amsterdam Neuroscience - Neurodegeneration, Amsterdam Neuroscience - Neuroinfection & -inflammation
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: The Dutch CPE surveillance Study Group 2021, ' National surveillance pilot study unveils a multicenter, clonal outbreak of VIM-2-producing Pseudomonas aeruginosa ST111 in the Netherlands between 2015 and 2017 ', Scientific Reports, vol. 11, no. 1, 21015 . https://doi.org/10.1038/s41598-021-00205-w
Scientific reports, 11(1):21015. Nature Publishing Group
Scientific Reports, 11(1):21015. Nature Publishing Group
Scientific Reports, 11:21015. Nature Publishing Group
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-021-00205-w
Popis: Verona Integron-encoded Metallo-beta-lactamase (VIM) is the most frequently-encountered carbapenemase in the healthcare-related pathogen Pseudomonas aeruginosa. In the Netherlands, a low-endemic country for antibiotic-resistant bacteria, no national surveillance data on the prevalence of carbapenemase-producing P. aeruginosa (CPPA) was available. Therefore, in 2016, a national surveillance pilot study was initiated to investigate the occurrence, molecular epidemiology, genetic characterization, and resistomes of CPPA among P. aeruginosa isolates submitted by medical microbiology laboratories (MMLs) throughout the country. From 1221 isolates included in the study, 124 (10%) produced carbapenemase (CIM-positive); of these, the majority (95, 77%) were positive for the blaVIM gene using PCR. Sequencing was performed on 112 CIM-positive and 56 CIM-negative isolates (n = 168), and genetic clustering revealed that 75/168 (45%) isolates were highly similar. This genetic cluster, designated Group 1, comprised isolates that belonged to high-risk sequence type ST111/serotype O12, had similar resistomes, and all but two carried the blaVIM-2 allele on an identical class 1 integron. Additionally, Group 1 isolates originated from around the country (i.e. seven provinces) and from multiple MMLs. In conclusion, the Netherlands had experienced a nationwide, inter-institutional, clonal outbreak of VIM-2-producing P. aeruginosa for at least three years, which this pilot study was crucial in identifying. A structured, national surveillance program is strongly advised to monitor the spread of Group 1 CPPA, to identify emerging clones/carbapenemase genes, and to detect transmission in and especially between hospitals in order to control current and future outbreaks.
Databáze: OpenAIRE
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