Reactor performance and microbial community dynamics during anaerobic biological treatment of wastewaters at 16-37 degrees C

Autor: Gavin Collins, Vincent O'Flaherty, Sharon McHugh, Micheal W. Carton
Rok vydání: 2009
Předmět:
Sucrose
t-rflp
RNA
Archaeal
Acetates
Applied Microbiology and Biotechnology
Methane
chemistry.chemical_compound
Bioreactors
RNA
Ribosomal
16S
Cluster Analysis
Anaerobiosis
environments
Phylogeny
16s rrna genes
Ecology
sediments
Chemical oxygen demand
Temperature
Biodiversity
Pulp and paper industry
RNA
Bacterial
DNA
Archaeal
psychrophilic anaerobic digestion
Methanomicrobiales
hydrogenotrophic methanogens
Water Microbiology
Anaerobic exercise
DNA
Bacterial
Molecular Sequence Data
Biology
microbial community dynamics
complex mixtures
Microbiology
DNA
Ribosomal
Water Purification
Biogas
Sequence Homology
Nucleic Acid
Bioreactor
Bacteria
technology
industry
and agriculture
Genes
rRNA
Sequence Analysis
DNA
equipment and supplies
biology.organism_classification
Fatty Acids
Volatile
Archaea
Anaerobic digestion
chemistry
Sludge
Zdroj: FEMS microbiology ecology. 48(3)
ISSN: 1574-6941
Popis: The anaerobic biological treatment of volatile fatty acid (VFA) - and sucrose - based wastewaters was investigated in two anaerobic bioreactors, R1 and R2, over a 300-day trial period. During the trial, the operating temperature of both reactors was lowered, in a stepwise fashion, from 37 to 16 degrees C. The VFA-fed reactor maintained an excellent level of performance, regardless of operating temperature, reaching COD removal efficiencies of 95% at 18 degrees C, and a biogas methane content in excess of 70% at 16 degrees C, at an imposed OLR of 20 kg COD m(-3) d(-1). However, an increase in the applied liquid upflow velocity to the bottom chamber of the reactor from 5 to 7.5 m h(-1)on day 236 resulted in a considerable decline in reactor performance. COD removal efficiencies in excess of 80% were achieved by the sucrose-fed reactor at 18 degrees C, at an imposed OLR of 20 kg COD m(-3) d(-1). An increase in the liquid upflow velocity applied to the sucrose-fed reactor resulted in enhanced reactor performance and stability, with respect to decreasing temperature. The different responses of both reactors to increased upflow velocity was associated with variations in the microbial population structure of the sludges, as determined by culture-independant molecular approaches, specifically the presence of high levels of delta-Proteobacteria and hydrogenotrophic methanogens in the VFA-fed biomass. High levels of Methanomicrobiales sp., in particular Methanocorpusculum parvum sp., were observed in both R1 and R2 during the trial. There was a distinct shift from acetoclastic methanogenic dominance to hydrogenotrophic dominance in both reactors in response to a decrease in the operating temperature.
Databáze: OpenAIRE
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