A Single-Nucleotide Synonymous Mutation in the gag Gene Controlling Human Immunodeficiency Virus Type 1 Virion Production
| Autor: | Takashi Okamoto, Shinji Irie, Yosio Mabuchi, Tsuyoshi Soji, Pathom Sawanpanyalert, Takaichi Hamano, Naoki Yamamoto, Naoko Takahashi, Yurina Hibi, Shudo Yamazaki, Takashi Hara, Ann Florence B. Victoriano, Hideki Yanai, Kazuhiro Matsuo |
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| Rok vydání: | 2007 |
| Předmět: |
Silent mutation
viruses Immunology Biology Microbiology Virus Virology Humans Cytotoxic T cell Nucleotide Gene Genetics chemistry.chemical_classification Nucleotides Structure and Assembly Virion virus diseases Group-specific antigen biology.organism_classification Genes gag chemistry Insect Science DNA Viral Mutation Lentivirus HIV-1 Viral disease |
| Zdroj: | Journal of Virology. 81:1528-1533 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Viral factors as well as host ones play major roles in the disease progression of human immunodeficiency virus type 1 (HIV-1) infection. We have examined cytotoxic T-lymphocyte activity and HIV-1 DNA PCR results of 312 high-risk seronegative drug users in northern Thailand and identified four seronegative cases positive for both assays. Furthermore, we have identified a synonymous mutation in nucleotide position 75 of the gag p17 gene (A426G) of HIV-1 that belongs to the CRF01_AE virus circulating in Thailand. The replication-competent HIV-1 clone containing the A426G mutation demonstrated a dramatic reduction of virion production and perturbation of viral morphogenesis without affecting viral protein synthesis in cells. |
| Databáze: | OpenAIRE |
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