Improved Multiplex PCR Strategy for Rapid Assignment of the Four Major Escherichia coli Phylogenetic Groups
| Autor: | M. J. Day, Michel Doumith, John Wain, Russell Hope, Neil Woodford |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Genetics Bacteriological Techniques Validation study Phylogenetic tree Bacteriology Biology Sequence types medicine.disease_cause Sensitivity and Specificity Molecular Diagnostic Techniques Multiplex polymerase chain reaction Escherichia coli medicine Humans Multilocus sequence typing Multiplex Polymerase Chain Reaction Escherichia coli Infections Sequence (medicine) |
| Zdroj: | Journal of Clinical Microbiology. 50:3108-3110 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data. |
| Databáze: | OpenAIRE |
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