Study of the solid phase extraction of pentoxifylline and its major metabolite as a basis of their rapid low concentration gas chromatographic determination in serum
| Autor: | Vladimír Marko, Katarína Bauerová |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pharmacology
Analyte Chromatography Gas Chromatography Chemistry Metabolite Clinical Biochemistry Extraction (chemistry) Alcohol General Medicine Biochemistry Analytical Chemistry Pentoxifylline chemistry.chemical_compound Capillary column Drug Discovery medicine Humans Solid phase extraction Molecular Biology Volume concentration medicine.drug |
| Zdroj: | Biomedical Chromatography. 5:256-261 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.1130050606 |
| Popis: | A gas-liquid chromatographic method for the determination of pentoxifylline and its secondary alcohol metabolite in serum has been developed. The method is based on the combination of solid phase extraction, capillary column separation and nitrogen-phosphorus detection of the analytes. Optimization of the solid phase extraction conditions permitted a low concentration determination, with limits of determination of 2 ng/mL and 10 ng/mL for pentoxifylline and its metabolite, respectively. The simplicity and rapidity of the extraction step was preserved. |
| Databáze: | OpenAIRE |
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