Vascular Pericytes Express Osteogenic Potential In Vitro and In Vivo
| Autor: | S. Walsh, Mary Jo Doherty, Ann E. Canfield, Jon N. Beresford, B A Ashton, Michael E. Grant |
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| Rok vydání: | 1998 |
| Předmět: |
Bone sialoprotein
Pathology medicine.medical_specialty Stromal cell Sialoglycoproteins Endocrinology Diabetes and Metabolism Cellular differentiation Osteocalcin Population In situ hybridization In Vitro Techniques Mice Osteogenesis medicine Animals Humans Integrin-Binding Sialoprotein Osteonectin Orthopedics and Sports Medicine RNA Messenger Osteopontin education Cells Cultured education.field_of_study Osteoblasts biology Stem Cells Retinal Vessels Cell Differentiation Antigens Differentiation Capillaries Cell biology medicine.anatomical_structure biology.protein Diffusion Chambers Culture Cattle Pericyte Stromal Cells Cell Division |
| Zdroj: | Journal of Bone and Mineral Research. 13:828-838 |
| ISSN: | 0884-0431 |
| DOI: | 10.1359/jbmr.1998.13.5.828 |
| Popis: | At postconfluence, cultured bovine pericytes isolated from retinal capillaries form three-dimensional nodule-like structures that mineralize. Using a combination of Northern and Southern blotting, in situ hybridization, and immunofluorescence we have demonstrated that this process is associated with the stage-specific expression of markers of primitive clonogenic marrow stromal cells (STRO-1) and markers of cells of the osteoblast lineage (bone sialoprotein, osteocalcin, osteonectin, and osteopontin). To demonstrate that the formation of nodules and the expression of these proteins were indicative of true osteogenic potential, vascular pericytes were also inoculated into diffusion chambers and implanted into athymic mice. When recovered from the host, chambers containing pericytes were found reproducibly to contain a tissue comprised of cartilage and bone, as well as soft fibrous connective tissue and cells resembling adipocytes. This is the first study to provide direct evidence of the osteogenic potential of microvascular pericytes in vivo. Our results are also consistent with the possibility that the pericyte population in situ serves as a reservoir of primitive precursor cells capable of giving rise to cells of multiple lineages including osteoblasts, chondrocytes, adipocytes, and fibroblasts. |
| Databáze: | OpenAIRE |
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