Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells
| Autor: | Yuangang Zu, Meng Luo, Yu-Jie Fu, Zuo-Fu Wei, Benjamin Wiench, Chang Gao, Cheng-Bo Gu, Onat Kadioglu, Thomas Efferth |
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| Rok vydání: | 2015 |
| Předmět: |
Cell cycle checkpoint
DNA damage Cell Pharmaceutical Science Apoptosis Biology Flow cytometry Cajanus Stilbenes Drug Discovery medicine Humans bcl-2-Associated X Protein Membrane Potential Mitochondrial Pharmacology medicine.diagnostic_test Caspase 3 Cell Cycle Checkpoints Cell cycle Molecular biology Salicylates Gene Expression Regulation Neoplastic medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Complementary and alternative medicine Cancer cell MCF-7 Cells Molecular Medicine DNA fragmentation DNA Damage Signal Transduction |
| Zdroj: | Phytomedicine. 22:462-468 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2015.02.005 |
| Popis: | Background: The low abundant cajanin stilbene acid (CSA) from Pigeon Pea ( Cajanus cajan ) has been shown to kill estrogen receptor α positive cancer cells in vitro and in vivo . Downstream effects such as cell cycle and apoptosis-related mechanisms have not been analyzed yet. Material and methods: We analyzed the activity of CSA by means of flow cytometry (cell cycle distribution, mitochondrial membrane potential, MMP), confocal laser scanning microscopy (MMP), DNA fragmentation assay (apoptosis), Western blotting (Bax and Bcl-2 expression, caspase-3 activation) as well as mRNA microarray hybridization and Ingenuity pathway analysis. Results: CSA induced G2/M arrest and apoptosis in a concentration-dependent manner from 8.88 to 14.79 µM. The MMP broke down, Bax was upregulated, Bcl-2 downregulated and caspase-3 activated. Microarray profiling revealed that CSA affected BRCA-related DNA damage response and cell cycle-regulated chromosomal replication pathways. Conclusion: CSA inhibited breast cancer cells by DNA damage and cell cycle-related signaling pathways leading to cell cycle arrest and apoptosis. |
| Databáze: | OpenAIRE |
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