Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer
| Autor: | Jinyun Wang, Zheng Gao, Qingxia Geng, Yinghui Zhao, Xiaoxia Liu, Xianhua Meng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research cervical cancer Cell Biology 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) Western blot microRNA medicine Cervical cancer medicine.diagnostic_test Oncogene matrix extracellular phosphoglycoprotein Articles General Medicine Cell cycle medicine.disease Molecular medicine 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis MEPE Cancer research miRNA-758 |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2017.4887 |
| Popis: | The present study aimed to examine the role and underlying mechanism of miRNA-758 (miR-758) expression in cancer tissues, blood and cervical exfoliated cells from patients with cervical cancer. A total of 49 patients with cervical cancer and 26 healthy people for cervical cancer screening were included in the present study. The patients with cervical cancer were treated with resection, and the tumor and adjacent tissues, blood and cervical exfoliated cells were collected. The expression levels of miR-758 and matrix extracellular phosphoglycoprotein (MEPE) mRNA in each sample were detected by reverse transcription-quantitative polymerase chain reaction. In addition, western blot analysis was used to detect the MEPE protein in tumor tissues, while ELISA was applied to detect the MEPE protein expression in the blood and cervical exfoliated cells. Compared with the normal control, MEPE mRNA expression was upregulated in cervical cancer tissues, blood and cervical exfoliated cells. At the protein level, MEPE was also upregulated significantly in patients with cervical cancer. miR-758 expression was decreased significantly in cervical cancer tissues, blood and cervical exfoliated cells (P |
| Databáze: | OpenAIRE |
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