Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea
| Autor: | M Beaudry, Mario Jacques, J M Fairbrother, J Harel, M S Donnenberg, C Zhu, Clarisse Desautels |
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| Rok vydání: | 1994 |
| Předmět: |
Diarrhea
Swine Immunology Virulence Enterotoxin Weaning Biology medicine.disease_cause Microbiology Pilus Bacterial Adhesion medicine Escherichia coli Animals Adhesins Bacterial Swine Diseases Escherichia coli Proteins biology.organism_classification Enterobacteriaceae Virology Bacterial adhesin Infectious Diseases Genes Bacterial Parasitology medicine.symptom Carrier Proteins Bacteria Bacterial Outer Membrane Proteins Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0019-9567 |
| Popis: | Escherichia coli O45 isolates associated with swine postweaning diarrhea in Québec were characterized with respect to virulence determinants genetically and investigated for their attaching and effacing (A/E) activities by experimental inoculation of gnotobiotic piglets and by the HEp-2 cell adherence assay. All of 32 isolates tested were negative for enterotoxigenic and verotoxigenic E. coli virulence determinants, heat-labile enterotoxin (LT), heat-stable enterotoxins (STap, STb), verotoxins (VT1, VT2), and F4 (K88), F5 (K99), F6 (987P), and F41, except one STb-positive and two F4-positive isolates. A total of 25 isolates hybridized with an EaeA probe, and 11 hybridized with an enteropathogenic E. coli adherence factor (EAF) probe. None of 32 isolates hybridized with a bundle-forming pilus (BFP) probe. The EAF, EaeA, and BFP factors have been associated with human enteropathogenic E. coli strains. A total of 10 of 12 eaeA-positive porcine O45 isolates induced A/E lesions characterized by intimate adherence of bacteria to the intestinal epithelial cell membrane with effacement of the microvilli, similar to those of human attaching-effacing E. coli. However, A/E lesions were not observed in the piglets inoculated with any one of three eaeA-negative O45 isolates. All E. coli O45 isolates were non-adherent to HEp-2 cells. Thus, we have demonstrated the production of typical A/E lesions by nonenterotoxigenic E. coli O45 isolates from swine postweaning diarrhea. The results indicate the significance of the eaeA gene in A/E activities of these isolates and suggest that EAF and BFP are not involved in O45 E. coli infection of weaning piglets. |
| Databáze: | OpenAIRE |
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