Out-of-equilibrium microcompartments for the bottom-up integration of metabolic functions
| Autor: | Dorothee Krafft, Kai Sundmacher, Ivan Ivanov, Jean-Christophe Baret, Thomas Beneyton, Tanja Vidaković-Koch, Christian Woelfer, Christin Kleineberg, Claudia Bednarz |
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| Přispěvatelé: | Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Max Planck Institute for Dynamics of Complex Technical Systems, Max-Planck-Gesellschaft, Otto-von-Guericke University [Magdeburg] (OVGU), ERC (FP7/2007- 2013/ERC Grant agreement 306385—SofI), by the ‘Région Aquitaine’ and by the French Government ‘Investments for the Future’ Programme, University of Bordeaux Initiative of Excellence (IDEX Bordeaux), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Metabolic state Science Microfluidics General Physics and Astronomy Glucose-6-Phosphate Nicotinamide adenine dinucleotide Glucosephosphate Dehydrogenase Gluconates Models Biological General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Bacterial microcompartment [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Active state lcsh:Science Multidisciplinary Artificial cell Bacteria Chemistry Cytoplasmic Vesicles General Chemistry NAD Metabolic pathway Kinetics 030104 developmental biology Biophysics lcsh:Q NAD+ kinase Metabolic activity Metabolic Networks and Pathways |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018) Nature Communications, Nature Publishing Group, 2018, 9, pp.2391. ⟨10.1038/s41467-018-04825-1⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-04825-1⟩ |
| Popis: | Self-sustained metabolic pathways in microcompartments are the corner-stone for living systems. From a technological viewpoint, such pathways are a mandatory prerequisite for the reliable design of artificial cells functioning out-of-equilibrium. Here we develop a microfluidic platform for the miniaturization and analysis of metabolic pathways in man-made microcompartments formed of water-in-oil droplets. In a modular approach, we integrate in the microcompartments a nicotinamide adenine dinucleotide (NAD)-dependent enzymatic reaction and a NAD-regeneration module as a minimal metabolism. We show that the microcompartments sustain a metabolically active state until the substrate is fully consumed. Reversibly, the external addition of the substrate reboots the metabolic activity of the microcompartments back to an active state. We therefore control the metabolic state of thousands of independent monodisperse microcompartments, a step of relevance for the construction of large populations of metabolically active artificial cells. |
| Databáze: | OpenAIRE |
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