Biodegradable Polymers and Gold Nanoparticle–Decorated Skin Substitutes: Synthesis, Characterization, and In Vitro Biological Activities
| Autor: | Santhiya Sasidharan, Lalitha Pottail |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
food.ingredient DPPH medicine.medical_treatment Metal Nanoparticles Nanoparticle Bioengineering Applied Microbiology and Biotechnology Biochemistry Gelatin Antioxidants Chitosan chemistry.chemical_compound food medicine Humans MTT assay Molecular Biology Skin Artificial integumentary system General Medicine Biodegradable polymer HEK293 Cells chemistry Colloidal gold Gold Biotechnology Nuclear chemistry |
| Zdroj: | Applied Biochemistry and Biotechnology. 193:3232-3252 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | Skin substitutes are a restorative material used to treat many skin injuries by replacing or masking the wound. It is also capable of producing an original skin type. In this study, gold nanoparticle-aided skin substitutes were prepared using biodegradable materials (chitosan, sodium alginate, and gelatin) under the magnetic stirring method. Gold ions were reduced using aqueous extract of Cyperus rotundus and Hemigraphis alternata. The formation of prepared gold nanoparticles was confirmed using spectroscopy techniques. The physical parameters of the skin substitutes were tested, and it was characterized using FTIR, DTG, laser profilometer, and FESEM analysis. HAaNP-aided skin substitutes have a bubble-like texture, and it facilitates higher water-absorbing ability. CRaNP aided skin substitutes reducing the hydrophilicity of the prepared skin substitutes. Antioxidant and antifungal skin substitute activities were carried out using DPPH radical scavenging activity and disk diffusion method, respectively. The antioxidant activity revealed the skin substitutes to possess significant free radical inhibition and as the number of gold nanoparticles increases, the activity also increases. The prepared samples show excellent activity against Aspergillus niger. The MTT assay reveals that the cancer cell (A-375) viability decreases by increasing skin substitutes' concentration. The normal cells (HEK-293) were cultured in a medium containing skin substitutes, facilitating the growth of cells. The cell attachment was observed in prepared cell lines after 24-h treatment. The results of this study suggest the prepared Cyperus rotundus and Hemigraphis alternata embedded with gold nanoparticle-aided skin substitutes are a promising material for medical and cosmetic application. |
| Databáze: | OpenAIRE |
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