Drosophila Damaged DNA Binding Protein 1 Contributes to Genome Stability in Somatic Cells
| Autor: | Masamitsu Yamaguchi, Kei Ichi Takata, Yoko Abe, Ryo Takeuchi, Kaori Shimanouchi, Shizuka Murakami, Tatsushi Ruike, Kengo Sakaguchi, Yoshihiro Kanai, Gen Ishikawa, Ryoichi Nakamura |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Genome instability animal structures Transgene fungi General Medicine Biology Biochemistry Molecular biology Genomic Instability Damaged DNA binding Animals Genetically Modified DNA-Binding Proteins Imaginal disc DDB1 RNA interference Animals Wings Animal Drosophila Female Molecular Biology Gene DNA Damage Nucleotide excision repair |
| Zdroj: | The Journal of Biochemistry. 139:51-58 |
| ISSN: | 1756-2651 0021-924X |
| Popis: | The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells. |
| Databáze: | OpenAIRE |
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