Human Embryonic Stem Cell Lines Derived from Discarded Embryos
| Autor: | Maisam Mitalipova, John D. Calhoun, Soojung Shin, Thomas C Schulz, Steven L. Stice, Ian Lyons, David Wininger, Allan J. Robins, Alison Venable, Scott Noggle |
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| Rok vydání: | 2003 |
| Předmět: |
KOSR
Somatic cell Cellular differentiation Basic fibroblast growth factor Embryoid body Biology Cell Line Mice chemistry.chemical_compound Animals Humans Embryo Disposition Stem Cells Cell Differentiation Cell Biology Embryo Mammalian Embryonic stem cell Molecular biology Cell biology Embryo Research chemistry Molecular Medicine Cattle Stem cell Biomarkers Stem Cell Transplantation Developmental Biology Adult stem cell |
| Zdroj: | Stem Cells. 21:521-526 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.21-5-521 |
| Popis: | Human pluripotent embryonic stem (ES) cells have important potential in regenerative medicine and as models for human preimplantation development; however, debate continues over whether embryos should be destroyed to produce human ES cells. We have derived four ES cell lines on mouse embryonic fibroblast cells in medium supplemented with basic fibroblast growth factor, human recombinant leukemia inhibitory factor, and fetal bovine serum. The source of these cell lines was poor-quality embryos that in the course of routine clinical practice would have been discarded. After continuous proliferation in vitro for more than 12 months, these ES cell lines maintained their developmental potential to form trophoblast and somatic cells, including cardiac muscle and neuronal tissue. |
| Databáze: | OpenAIRE |
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