Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation
| Autor: | Rupert M. Bruckmaier, Lorenzo E. Hernández-Castellano, E. L. Endres, Allan S. Prichard, Noah L. Maerz, Samantha R. Weaver, M.S. Akins, Laura L. Hernandez, Austin P Prichard |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Blood Glucose
0301 basic medicine Physiology Peptide Hormones medicine.medical_treatment lcsh:Medicine Fatty Acids Nonesterified Biochemistry Energy homeostasis Endocrinology Reproductive Physiology Pregnancy Lactation Receptor Serotonin 5-HT2B Medicine and Health Sciences Homeostasis Insulin lcsh:Science reproductive and urinary physiology 2. Zero hunger Multidisciplinary 630 Agriculture Organic Compounds Caspase 3 Monosaccharides Postpartum Period food and beverages Neurochemistry Neurotransmitters 04 agricultural and veterinary sciences Body Fluids Chemistry Blood medicine.anatomical_structure Liver Hepatocyte Physical Sciences 590 Animals (Zoology) Female Anatomy Research Article Signal Transduction Biogenic Amines Serotonin medicine.medical_specialty Transmembrane Receptors Carbohydrates Biology Glucagon 03 medical and health sciences Internal medicine medicine Animals Diabetic Endocrinology Calcium metabolism Endocrine Physiology Organic Chemistry lcsh:R Chemical Compounds 0402 animal and dairy science Biology and Life Sciences Proteins Cyclin-Dependent Kinase 4 Cell Biology 040201 dairy & animal science Hormones Glucose Ki-67 Antigen 030104 developmental biology Cattle lcsh:Q Physiological Processes Neuroscience Serotonin Receptors |
| Zdroj: | Weaver, Samantha R; Prichard, Allan S; Maerz, Noah L; Prichard, Austin P; Endres, Elizabeth Lynne; Hernandez Castellano, Lorenzo Enrique; Akins, Matthew S; Bruckmaier, Rupert; Hernandez, Laura L (2017). Elevating serotonin pre-partum alters the Holstein dairy cow hepatic adaptation to lactation. PLoS ONE, 12(9), e0184939. Public Library of Science 10.1371/journal.pone.0184939 PLoS ONE, Vol 12, Iss 9, p e0184939 (2017) PLoS ONE |
| DOI: | 10.1371/journal.pone.0184939 |
| Popis: | Serotonin is known to regulate energy and calcium homeostasis in several mammalian species. The objective of this study was to determine if pre-partum infusions of 5-hydroxytryptophan (5-HTP), the immediate precursor to serotonin synthesis, could modulate energy homeostasis at the level of the hepatocyte in post-partum Holstein and Jersey dairy cows. Twelve multiparous Holstein cows and twelve multiparous Jersey cows were intravenously infused daily for approximately 7 d pre-partum with either saline or 1 mg/kg bodyweight of 5-HTP. Blood was collected for 14 d post-partum and on d30 post-partum. Liver biopsies were taken on d1 and d7 post-partum. There were no changes in the circulating concentrations of glucose, insulin, glucagon, non-esterified fatty acids, or urea nitrogen in response to treatment, although there were decreased beta-hydroxybutyrate concentrations with 5-HTP treatment around d6 to d10 post-partum, particularly in Jersey cows. Cows infused with 5-HTP had increased hepatic serotonin content and increased mRNA expression of the serotonin 2B receptor on d1 and d7 post-partum. Minimal changes were seen in the hepatic mRNA expression of various gluconeogenic enzymes. There were no changes in the mRNA expression profile of cell-cycle progression marker cyclin-dependent kinase 4 or apoptotic marker caspase 3, although proliferating cell nuclear antigen expression tended to be increased in Holstein cows infused with 5-HTP on d1 post-partum. Immunofluorescence assays showed an increased number of CASP3- and Ki67-positive cells in Holstein cows infused with 5-HTP on d1 post-partum. Given the elevated hepatic serotonin content and increased mRNA abundance of 5HTR2B, 5-HTP infusions may be stimulating an autocrine-paracrine adaptation to lactation in the Holstein cow liver. |
| Databáze: | OpenAIRE |
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