The inclusion complexes of hesperetin and its 7-rhamnoglucoside with (2-hydroxypropyl)-β-cyclodextrin
| Autor: | Chiara Costa, Stefania Catania, Valentina Villari, Paola Donato, Silvana Tommasini, Rosanna Stancanelli, Calabrò Ml, Paola Ficarra, Rita Ficarra |
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| Rok vydání: | 2005 |
| Předmět: |
UV-vis spectroscopy
(2-Hydroxypropyl)-β-cyclodextrin Time Factors Drug Industry Ultraviolet Rays Chemistry Pharmaceutical Iron Clinical Biochemistry Pharmaceutical Science Analytical Chemistry Inclusion compound chemistry.chemical_compound Hesperidin Glucosides Biological assays Computational chemistry Drug Discovery Hesperetin Organic chemistry Solubility Fluorescence spectroscopy Spectroscopy Hydroxyl Radical beta-Cyclodextrins Water Solubility measurements Molecular encapsulation Binding constant 2-Hydroxypropyl-beta-cyclodextrin Oxygen Spectrometry Fluorescence Aglycone Microscopy Fluorescence Models Chemical cyclodextrin chemistry Spectrophotometry Flavanones Pharmaceutics Spectrophotometry Ultraviolet Comet Assay Protein Binding |
| Zdroj: | Journal of pharmaceutical and biomedical analysis 39 (2005): 572–580. doi:10.1016/j.jpba.2005.05.009 info:cnr-pdr/source/autori:S. Tommasini, M.L. Calabrò, R. Stancanelli, P. Donato, C. Costa, S. Catania, V. Villari, P. Ficarra, R. Ficarra/titolo:The inclusion complexes of hesperetin and its 7-rhamnoglucoside with (2-hydroxypropyl)-beta-cyclodextrin/doi:10.1016%2Fj.jpba.2005.05.009/rivista:Journal of pharmaceutical and biomedical analysis (Print)/anno:2005/pagina_da:572/pagina_a:580/intervallo_pagine:572–580/volume:39 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2005.05.009 |
| Popis: | The effect of (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CyD) on the solubility properties and spectroscopic features of hesperetin and its 7-rhamnoglucoside, hesperidin, was qualitatively and quantitatively investigated in water, by means of UV-vis absorption and fluorescence spectroscopy. The stoichiometric ratios and stability constants describing the extent of formation of the complexes have been determined by phase-solubility measurements; in both cases type-A(L) diagrams have been obtained (soluble 1:1 complexes). The higher degree of interaction showed by hesperetin may be attributed to the higher hydrophobicity and smaller size of the aglycone molecule, which therefore exhibits a greater affinity for the CyD and fits better into the cavity. The effect of molecular encapsulation on the two flavanones antioxidant activity was afterwards evaluated by means of different biological assays, concerned to the different mechanisms of in vivo action. The protection efficacy was in all cases higher for the complexed drugs, with respect to the free ones; these results are of great interest for their potential usefulness in pharmaceutics. |
| Databáze: | OpenAIRE |
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