MicroRNA-424 regulates cisplatin resistance of gastric cancer by targeting SMURF1 based on GEO database and primary validation in human gastric cancer tissues
| Autor: | Li Lu, Menglin Wu, Yaoheng Lu, Tong Liu, Weihua Fu, Weidong Li, Zhicheng Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cisplatin Microarray gastric cancer Human Protein Atlas Cancer Biology medicine.disease cisplatin drug resistance Fold change OncoTargets and Therapy 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology 030220 oncology & carcinogenesis microRNA miRNAs medicine Cancer research Pharmacology (medical) KEGG Gene medicine.drug Original Research |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| Popis: | Li Lu,1 Menglin Wu,2 Yaoheng Lu,1 Zhicheng Zhao,1 Tong Liu,1 Weihua Fu,1 Weidong Li1 1Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People’s Republic of China; 2Radiology Department, Second Hospital of Tianjin Medical University, Tianjin, People’s Republic of ChinaCorrespondence: Weidong LiDepartment of General Surgery, Tianjin Medical University General Hospital, No.154, Anshan Road, Heping District, Tianjin, People’s Republic of ChinaTel +86 226 036 3901Fax +86 226 036 3901Email tjmughgs_lwd@163.comPurpose: Cisplatin (DDP) based chemotherapy regimens are widely used in advanced gastric cancer (GC). Drug resistance often limited the clinical benefits of cisplatin regimen. The mechanisms of cisplatin resistance have not been fully revealed. Therefore, further exploration of the relevant molecular mechanisms is urgently needed.Patients and methods: DDP resistance associated miRNA of GC microarray dataset GSE86195 was obtained from the National Center for Biotechnology Information (NCBI) GEO database, GEO2R was applied to compare the samples in two different groups under the same experimental conditions. |log2(Fold Change) | (log2(FC)) was selected as the criteria to screen the statistically significant DE-miRNAs. StarBaseV3.0 was used to predict the target genes of the DE-miRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of target genes of DE-miRNAs were carried out using DAVID. The STRING database was applied to estimate the correlations between target genes. Analysis of hubgenes by coremine and The Human Protein Atlas (THPA). Initial expression validations of miR-424 and miR-491-5p, SMURF1 and BCL2L1 were carried out using clinical pathological specimens by RT-PCR.Results: A total of 13 Differential expression-miRNAs (DE-miRNAs) were identified in DDP chemoresistant cells, including 9 upregulated miRNAs and 4 downregulated miRNAs. SMURF1 and BCL2L1 were screened as the critical genes in DDP-resistant GC, which were regulated by miR-424 and miR-491-5p respectively. The results of validation of hub genes expression in GC tissues indicated that in DFS |
| Databáze: | OpenAIRE |
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