Terminal sterilization of BisGMA-TEGDMA thermoset materials and their bioactive surfaces by supercritical CO2

Autor: Dario Solinas, Ivan Donati, Monica Benincasa, Sara Spilimbergo, Sergio Paoletti, Gianluca Turco, Ireneo Kikic, Marie-Pierre Foulc, Mila Toppazzini
Přispěvatelé: Donati, Ivan, Benincasa, Monica, Foulc, M. P., Turco, Gianluca, Toppazzini, M., Solinas, Dario, Spilimbergo, S., Kikic, Ireneo, Paoletti, Sergio
Rok vydání: 2012
Předmět:
Sterility assurance
Staphylococcus aureus
Materials science
Silver
Polymers and Plastics
Surface Properties
Silver nanoparticle
Thermosetting polymer
Metal Nanoparticles
Bioengineering
Biocompatible Materials
Microbial Sensitivity Tests
Sterilization proce
Polyethylene Glycols
Biomaterials
Bioactive surfaces
Fiber-reinforced composite
Low temperatures
Silver nanoparticles
Sterilization process
Supercritical CO
Surface hardness
Thermoset materials
Unreacted monomers
Structure-Activity Relationship
Polymethacrylic Acids
Polysaccharides
Polymer chemistry
Materials Chemistry
Low temperature
Bisphenol A-Glycidyl Methacrylate
Surface hardne
Sterility assurance level
Thermoset material
Chromatography
High Pressure Liquid
Dose-Response Relationship
Drug
Temperature
Terminal Sterilization
Hydrogen Peroxide
Carbon Dioxide
Supercritical fluid
Bioactive surface
Anti-Bacterial Agents
Chemical engineering
Zdroj: Biomacromolecules. 13(4)
ISSN: 1526-4602
Popis: The development of biomaterials endowed with bioactive features relies on a simultaneous insight into a proper terminal sterilization process. FDA recommendations on sterility of biomaterials are very strict: a sterility assurance level (SAL) of 10(-6) must be guaranteed for biomaterials to be used in human implants. In the present work, we have explored the potential of supercritical CO(2) (scCO(2)) in the presence of H(2)O(2) as a low-temperature sterilization process for thermoset materials and their bioactive surfaces. Different conditions allowing for terminal sterilization have been screened and a treatment time-amount of H(2)O(2) relationship proposed. The selected terminal sterilization conditions did not notably modify the mechanical properties of the thermoset nor of their fiber-reinforced composites. This was confirmed by μCT analyses performed prior to and after the treatment. On the contrary, terminal sterilization in the presence of H(2)O(2) induced a slight decrease in the surface hardness. The treatment of the thermoset material with scCO(2) led to a reduction in the residual unreacted monomers content, as determined by means of high performance liquid chromatography (HPLC) analyses. Finally, it was found that a thermoset coated with a polysaccharide layer containing silver nanoparticles maintained a very high antimicrobial efficacy even after the scCO(2)-based terminal sterilization.
Databáze: OpenAIRE
Externí odkaz: