Disparate thermostability profiles and HN gene domains of field isolates of Newcastle disease virus from live bird markets and waterfowl in Uganda
| Autor: | Kizito K. Mugimba, Denis K. Byarugaba, John Bosco Omony, Agnes Wanyana, Jessica L. Nakavuma, Maxwell Otim-Onapa, Halid Kirunda |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Hot Temperature 040301 veterinary sciences Newcastle Disease Newcastle disease virus Virulence Biology Newcastle disease Microbiology Birds 0403 veterinary science Viral Proteins 03 medical and health sciences Protein Domains Anseriformes Hemagglutionation assay Virology Animals Uganda Thermolabile Thermostability Infectivity HN Protein Bird Diseases Protein Stability Research Thermostable isolate HN gene Embryonated Outbreak 04 agricultural and veterinary sciences biology.organism_classification 030104 developmental biology Infectious Diseases Enzootic |
| Zdroj: | Virology Journal |
| ISSN: | 1743-422X |
| DOI: | 10.1186/s12985-016-0560-0 |
| Popis: | Background Uganda poultry production is still faced with frequent outbreaks of Newcastle disease (ND) in the backyard free-range systems despite the accessibility of cross protective vaccines. Live bird markets and waterfowl has long been reported as a major source of disease spread as well as potential sources of avirulent strains that may mutate to virulent strains. ND-virus has been reported enzootic in Ugandan poultry but limited studies have been conducted to ascertain thermostability phenotypes of the Ugandan ND-virus strains and to understand how these relate to vaccine strains. Methods This study evaluated thermostability of 168 ND-virus field isolates recovered from live bird markets and waterfowls in Uganda compared to two live commercial vaccine strains (I2 and LaSota) by standard thermostability procedures and Hemagglutinin-Neuraminidase (HN) gene domains. The known pathotypes with thermostability profiles were compared at HN amino acid sequences. Results Field isolates displayed disparate heat stability and HN gene domains. Thermolabile isolates were inactivated within 15 min, while the most thermostable isolates were inactivated in 120 min. Four thermostable isolates had more than 2 log2 heamaglutinin (HA) titers during heat treatment and the infectivity of 9.8 geometric mean of log10 EID50 % in embryonated eggs. One isolate from this study exhibited a comparable thermostability and stable infectivity titers after serial passages, to that of reference commercial vaccine was recommended for immunogenicity and protection studies. Conclusion The occurrence of ND-virus strains in waterfowl and live bird markets with disparate thermostability and varying HN gene domains indicate circulation of different thermostable and thermolabile ND-virus pathotypes in the country. |
| Databáze: | OpenAIRE |
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