Enhanced bioactivity of Zanthoxylum schinifolium fermented extract: Anti-inflammatory, anti-bacterial, and anti-melanogenic activity
| Autor: | Jeong-Muk Lim, Byung-Taek Oh, Yool-Jin Park, Kamala-Kannan Seralathan, Se-Won Lee, Jeong-Ho Lee, Harshavardhan Mohan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Zanthoxylum
0106 biological sciences 0301 basic medicine medicine.drug_class Tyrosinase Anti-Inflammatory Agents Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Gas Chromatography-Mass Spectrometry Anti-inflammatory Cell Line Mice Starfish 03 medical and health sciences Bioreactors Lactobacillus rhamnosus 010608 biotechnology Staphylococcus epidermidis medicine Animals Humans Viability assay Food science Enzyme Inhibitors Cytotoxicity Melanins biology Lacticaseibacillus rhamnosus Monophenol Monooxygenase Plant Extracts Chemistry Macrophages food and beverages Skin whitening biology.organism_classification Anti-Bacterial Agents RAW 264.7 Cells 030104 developmental biology Fermentation Biotechnology Zanthoxylum schinifolium |
| Zdroj: | Journal of Bioscience and Bioengineering. 129:638-645 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2019.12.003 |
| Popis: | Fermented extracts have evolved to be a potential alternative to synthetic chemicals, owing to their anti-inflammatory and anti-bacterial properties. This study intends to assess the potential of fermented Zanthoxylum schinifolium extract for use in biomedical applications. Probiotic bacteria, Lactobacillus rhamnosus A6-5, were deployed as a seed culture for fermentation. The fermented extract showed greater tyrosinase inhibitory activity and reduced melanin production (58.3%) compared with the raw extract. Cytotoxicity assay inferred that 500 μg/mL is the ideal non-toxic concentration with maximum cell viability. In addition, DAPI staining did not show any damage to the chromatin structure of the cells. The anti-aging property of the fermented extract was confirmed by a decrease in IL-6 content. The fermented extract showed lower MIC (40 mg/mL) and MBC (60 mg/mL), indicating greater anti-bacterial activity than the raw extract. The results confirmed that the fermented Z. schinifolium extract has high biomedical properties compared with the raw extract and can be used as an ideal skin whitening agent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect