Selective monitoring of vitamin D2 and D3 supplementation with a highly specific 25-hydroxyvitamin D3 immunoassay with negligible cross-reactivity to 25-hydroxyvitamin D2
| Autor: | William D. Fraser, Hans Christian Hoeck, Mario Taranto, Paul Glendenning, Dennis Bang Henriksen, Samuel D Vasikaran, Bo Li, Per Qvist, Inger Byrjalsen, Claus Christiansen |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Vitamin medicine.medical_specialty Denmark Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Cross Reactions medicine.disease_cause Sensitivity and Specificity Biochemistry Cross-reactivity chemistry.chemical_compound Internal medicine medicine Vitamin D and neurology Humans Aged Calcifediol Cholecalciferol 25-Hydroxyvitamin D 2 Aged 80 and over Postmenopausal women medicine.diagnostic_test business.industry Biochemistry (medical) General Medicine Middle Aged medicine.disease Menopause Ergocalciferol Endocrinology chemistry Immunoassay Dietary Supplements Ergocalciferols Female Drug Monitoring business medicine.drug |
| Zdroj: | Clinica Chimica Acta. 404:144-148 |
| ISSN: | 0009-8981 |
| Popis: | Background: The effects of vitamin D 2 and D 3 supplementation on circulating concentrations of 25(OH)D 3 require reliable analytical tools for specific determination of 25(OH)D 3 and 25(OH)D 2 . We have developed a highly specific 25-OH Vitamin D3 ELISA with negligible cross-reactivity towards 25(OH)D 2 . Methods: 25(OH)D 3 concentrations were measured in several study participants; 1) 641 healthy men and women; 2) 39 postmenopausal women receiving 400-800 IU vitamin D 3 daily for 4 months; 3) 45 men and women with hip fracture receiving 1000 IU vitamin D 2 daily for 3 months. Results: This 25-OH Vitamin D3 ELISA had minimal cross-reactivity to 25(OH)D 2 , (0.7%). and demonstrated a high correlation (r 2 = 0.93) with 25(OH)D 3 determined by HPLC. 25(OH)D 3 increased by 14% in subjects receiving vitamin D 3 for 4 months (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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