An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis
| Autor: | C. E. Stevens, A. R. Neurath, E. J. Harley, W. R. Oleszko, N. Strick, W. Szmuness |
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| Rok vydání: | 1980 |
| Předmět: |
Male
Hepatitis Viral Human Radioimmunoassay Blood Donors Centrifugation Isopycnic Biology Blood serum Antigen Virology medicine Humans Isoelectric Point Antigens Hepatitis Alanine Transaminase Bilirubin Hepatitis C Hepatitis A Hepatitis B medicine.disease Molecular biology Molecular Weight Alanine transaminase biology.protein Antibody |
| Zdroj: | Journal of General Virology. 48:285-295 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-48-2-285 |
| Popis: | In a search for additional antigens associated with virus-induced human liver disease a radioimmunoassay (RIA) was developed using IgG from sera of a multiply transfused person. Polystyrene beads coated with IgG F(ab)'2 fragments, dinitrophenylated F(ab)'2 fragments and 125I-labelled anti-2,4-dinitrophenyl antibodies (Neurath & Strick, 1979) were used in the RIA. An apparently new antigen or the corresponding antibodies were detected in 155 serum specimens from 35/37 (94%)individuals who developed non-A, non-B hepatitis. The antigen was also present in hepatitis B surface antigen-negative sera of blood donors with normal (13.2%) and elevated levels of alanine aminotransferase (34%). The antigen has an approximate mol. wt. of 45,000, a buoyant density of 1.23 g/ml and an isoelectric point of 7. |
| Databáze: | OpenAIRE |
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