Lactobacillus delivery of bioactive interleukin-22
| Autor: | Harold Marcotte, Kasper Krogh-Andersen, Yin Lin, Lennart Hammarström |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology GVHD Graft vs Host Disease Bioengineering Applied Microbiology and Biotechnology Cell Line Interleukin 22 Mice 03 medical and health sciences Intestinal mucosa Lactobacillus Animals Humans Secretion Intestinal Mucosa biology Research Interleukins Regeneration (biology) food and beverages Lacticaseibacillus paracasei Flow Cytometry biology.organism_classification Recombinant Proteins Interleukin-10 Transplantation Mouse interleukin-22 030104 developmental biology Cell culture Immunology Stem cell Lactobacillus paracasei BL23 Plasmids Biotechnology |
| Zdroj: | Microbial Cell Factories |
| ISSN: | 1475-2859 |
| DOI: | 10.1186/s12934-017-0762-1 |
| Popis: | Background Interleukin-22 (IL-22) plays a prominent role in epithelial regeneration and dampening of chronic inflammatory responses by protecting intestinal stem cells from immune-mediated tissue damage. IL-22 has a considerable therapeutic potential in graft-versus-host disease (GVHD), which is a frequent and challenging complication following allogeneic stem cell transplantation. The aim of our study was to engineer Lactobacillus for delivery of IL-22 directly to the intestinal mucosa as a new therapeutic strategy for GVHD. Results The secretion and surface anchoring of mouse IL-22 by Lactobacillus paracasei BL23 was demonstrated by Western blot and flow cytometry. Both secreted and anchored mouse IL-22 produced by Lactobacillus was biologically active, as determined by its ability to induce IL-10 secretion in the Colo 205 human colon cancer cell line. Conclusions We have demonstrated the secretion and surface anchoring of bioactive IL-22 by Lactobacillus. Our results suggest that IL-22 expressing lactobacilli may potentially be a useful mucosal therapeutic agent for the treatment of GVHD, provided that chromosomal integration of the IL-22 expression cassettes can be achieved. Electronic supplementary material The online version of this article (doi:10.1186/s12934-017-0762-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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