Biotin formation by recombinant strains of Escherichia coli: influence of the host physiology
| Autor: | S.W. Brown, M. O'Regan, L. Caussin, J. Reymund, C. Hebert, D. Weltin, J. Sabatié, C. Ledoux, K. Kamogawa, Denis Speck, Yves Lemoine, Remi Gloeckler, I. Ohsawa, S. Bernard |
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| Rok vydání: | 1991 |
| Předmět: |
Biotin
Bioengineering Bacillus General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Bacillus sphaericus Fed-batch culture Culture Media chemistry.chemical_compound Pimelic acid chemistry Biochemistry medicine Bioreactor Escherichia coli Vitamer Transformation Bacterial Bacteria Biotechnology Plasmids |
| Zdroj: | Journal of biotechnology. 20(1) |
| ISSN: | 0168-1656 |
| Popis: | Strains of Escherichia coli were transformed with different plasmids bearing the gene clusters bioXWF and bioDAYB isolated from the Gram positive bacterium Bacillus sphaericus . These genes encode for the enzymes involved in the metabolic pathway which synthesizes biotin from the precursor pimelic acid. Transformed E. coli strains were grown in bioreactors to reach a biomass of 18 g l −1 cell dry weight in 1 litre batch culture with substrate feeding and approximately 50 g l −1 in 10 l fed batch culture. Improved yields of total vitamers and biotin formed in these processes were achieved after a comparative analysis of different culture conditions, medium compositions, host strains and expression systems. Production of 27 mg l −1 of biotin and 200 mg 1 −1 of vitamers was achieved in 1 litre batch culture. Using a 10-1 fed batch process, biotin and vitamer concentrations reached maximum values of 45 mg l −1 and 350 mg l −1 respectively. |
| Databáze: | OpenAIRE |
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