Effect of concanavalin A and its succinylated derivative on the oxidative metabolism of guinea pig peritoneal macrophages
| Autor: | Takeshi Kambara, Tatsuomi Yasaka |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Guinea Pigs
Biophysics Pentose phosphate pathway Biochemistry Divalent Guinea pig Receptors Concanavalin A Structure-Activity Relationship Oxygen Consumption Concanavalin A Macrophage Animals Peritoneal Cavity Glycoproteins chemistry.chemical_classification biology Dose-Response Relationship Drug Chemistry Macrophages Cell Membrane Lectin Cell Biology Transmembrane protein biology.protein Glycoprotein |
| Zdroj: | Biochimica et biophysica acta. 508(2) |
| ISSN: | 0006-3002 |
| Popis: | The effect of concanavalin A and its succinylated derivative on the metabolic regulation of guinea pig peritoneal macrophages was observed. The binding of tetravalent concanavalin A to the surface glycoproteins of macrophages caused a marked increase in the rate of oxygen consumption due to the activation of the hexose monophosphate shunt. Divalent succinylated concanavalin A, also induced a similar change in the rate of oxygen metabolism. The metabolic change induced by these two types of lectin was reversibly inhibited by α-methyl- d -glucoside, a haptenic inhibitor of these lectins, and was temperature dependent (observed at above 15°C). It is suggested that the binding of these lectins to the surface glycoproteins, and not their cross-linking into caps, is required for the activation of oxygen metabolism of macrophages, and that highly fluid state of the plasma membrane seems to be an essential requirement for the transduction of glycoprotein perturbation on the macrophage surface into cellular interior via transmembrane control mechanism. |
| Databáze: | OpenAIRE |
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