Localization of neuronal-constitutive nitric oxide synthase and secretory regulation by nitric oxide in the rat submandibular and sublingual glands
| Autor: | Kyoko Higuchi, Yo Yoshida, Kenji Uchihashi, Masaki Yamaguchi, Noriyasu Takai |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Superior cervical ganglion Submandibular Gland Superior Cervical Ganglion Nitric Oxide Benzoates Second Messenger Systems Nitric oxide Sublingual Gland chemistry.chemical_compound Nerve Fibers stomatognathic system Internal medicine medicine Animals Secretion Enzyme Inhibitors Rats Wistar General Dentistry Neurons Dose-Response Relationship Drug biology Salivary gland Imidazoles Sublingual gland Ganglia Parasympathetic Free Radical Scavengers Cell Biology General Medicine Submandibular ganglion Immunohistochemistry Submandibular gland Electric Stimulation Rats Nitric oxide synthase NG-Nitroarginine Methyl Ester medicine.anatomical_structure Endocrinology Otorhinolaryngology chemistry biology.protein Chorda Tympani Nerve Nitric Oxide Synthase |
| Zdroj: | Archives of Oral Biology. 44:745-750 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/s0003-9969(99)00064-3 |
| Popis: | The distribution of neuronal-constitutive nitric oxide synthase (ncNOs)-positive nerve fibres was compared immunohistochemically, and the effect of NOs inhibitor and NO scavenger on the secretory response was compared functionally, in the two glands. Numerous ncNOs-positive fibres were distributed around acini in the submandibular gland but scarcely any around acini in the sublingual gland. Within the submandibular ganglion (parasympathetic), the nerve-cell bodies were strongly positive. Within the superior cervical ganglion (sympathetic), the nerve-cell bodies were negative, although some positive nerve fibres were observed. The secretory responses to the electrical stimulation of the chorda were significantly reduced by the NOs inhibitor, NG-nitro- l -arginine methyl ester (L-NAME, 10−9–10−3 M) in a dose-dependent manner. The NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (carboxy-PTIO) also reduced the chorda-evoked secretion (10−9–10−6 M). The submandibular secretions evoked by stimulation of the superior cervical ganglion were not affected by L-NAME or carboxy-PTIO. In the sublingual gland, neither L-NAME nor carboxy-PTIO affected chorda-evoked salivary secretion. The histochemical and functional results both suggest that NO plays an excitatory role in the regulation of parasympathetic nerve-induced salivary secretion in the rat submandibular gland, but not in the sublingual gland. |
| Databáze: | OpenAIRE |
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