The use of heparin chemistry to improve dental osteogenesis associated with implants
| Autor: | Donghyun Lee, Dong Nyoung Heo, Joohyoung Kim, Wan Doo Kim, Sang Jin Lee, Min Soo Bae, Il Keun Kwon, Deok Won Lee, Min Heo, Seoung-Jin Hong, Su A Park |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
In vitro test
Polymers and Plastics Periodontal ligament stem cells Periodontal Ligament Surface Properties 02 engineering and technology 03 medical and health sciences 0302 clinical medicine Growth Differentiation Factor 5 Osteogenesis In vivo Materials Chemistry medicine Animals Humans Bone formation Cells Cultured Dental Implants Titanium Heparin Chemistry Stem Cells Organic Chemistry Cell Differentiation 030206 dentistry 021001 nanoscience & nanotechnology embryonic structures Rabbit model Surface modification Rabbits 0210 nano-technology Quantitative analysis (chemistry) Biomedical engineering medicine.drug |
| Zdroj: | Carbohydrate Polymers. 157:1750-1758 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2016.11.062 |
| Popis: | In this study, we designed a hybrid Ti by heparin modifying the Ti surface followed by Growth/differentiation factor-5 (GDF-5) loading. After that, products were characterized by physicochemical analysis. Quantitative analysis of functionalized groups was also confirmed. The release behavior of GDF-5 grafted samples was confirmed for up to 21days. The surface modification process was found to be successful and to effectively immobilize GDF-5 and provide for its sustained release behavior. As an in vitro test, GDF-5 loaded Ti showed significantly enhanced osteogenic differentiation with increased calcium deposition under nontoxic conditions against periodontal ligament stem cells (PDLSc). Furthermore, an in vivo result showed that GDF-5 loaded Ti had a significant influence on new bone formation in a rabbit model. These results clearly confirmed that our strategy may suggest a useful paradigm by inducing osseo-integration as a means to remodeling and healing of bone defects for restorative procedures in dentistry. |
| Databáze: | OpenAIRE |
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