Atividade diferencial in vitro de fosfolipases e proteinases ácidas de isolados clínicos de Candida
| Autor: | Patrícia de Maria Silva Figueiredo, Silvio Gomes Monteiro, Cristina de Andrade Monteiro, Fernanda Costa Rosa, Anderson França da Silva, Aurean D'Eça Júnior |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 medicine.medical_treatment Virulence Phospholipase Biology Microbiology Proteinases Extracellular medicine Animals Humans Bovine serum albumin Candida chemistry.chemical_classification Protease Fatores de virulência Virulence factors Proteases In vitro Corpus albicans Fosfolipases Infectious Diseases Enzyme chemistry Phospholipases biology.protein Parasitology Cattle Peptide Hydrolases |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical v.44 n.3 2011 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 334-338 (2011) Revista da Sociedade Brasileira de Medicina Tropical, Volume: 44, Issue: 3, Pages: 334-338, Published: 10 JUN 2011 |
| ISSN: | 1678-9849 |
| Popis: | INTRODUCTION: Candida yeasts are commensals; however, if the balance of normal flora is disrupted or the immune defenses are compromised, Candida species can cause disease manifestations. Several attributes contribute to the virulence and pathogenicity of Candida, including the production of extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate the in vitro activity of phospholipases and acid proteinases in clinical isolates of Candida spp. METHODS: Eighty-two isolates from hospitalized patients collected from various sites of origin were analyzed. Phospholipase production was performed in egg yolk medium and the production of proteinase was verified in a medium containing bovine serum albumin. The study was performed in triplicate. RESULTS: Fifty-six (68.3%) of isolates tested were phospholipase positive and 16 (44.4%) were positive for proteinase activity. C. tropicalis was the species with the highest number of positive isolates for phospholipase (91.7%). Statistically significant differences were observed in relation to production of phospholipases among species (p |
| Databáze: | OpenAIRE |
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