Evolution of foamy macrophages in the pulmonary granulomas of experimental tuberculosis models
| Autor: | Olga Gil, Gustavo Tapia, Sergio Pinto, Pere-Joan Cardona, Cristina Vilaplana, Neus Cáceres, Frédéric Altare, Isabel Ojanguren |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Tuberculosis Guinea Pigs Immunology Biology Microbiology Mycobacterium tuberculosis Mice Necrosis Phagosomes Macrophages Alveolar medicine Animals Electronic microscopy Pulmonary pathology Lung Tuberculosis Pulmonary Granuloma medicine.disease biology.organism_classification Experimental tuberculosis Mice Inbred C57BL Disease Models Animal Microscopy Electron Cholesterol Infectious Diseases Mice Inbred DBA Chronic Disease Disease Progression Female Intracellular Foamy macrophages Foam Cells |
| Zdroj: | Tuberculosis. 89:175-182 |
| ISSN: | 1472-9792 |
| DOI: | 10.1016/j.tube.2008.11.001 |
| Popis: | The chronic phase of Mycobacterium tuberculosis infection in mouse experimental models is characterized by the accumulation of foamy macrophages (FM)--which shape the outer ring of the granuloma - in the alveolar spaces, as detected in paraffin-embedded tissues stained with hematoxylin-eosin. In this study, the use of semi- and ultra-thin sections offers more detailed information about the origin of FM both in mouse and guinea-pig experimental models. Lipid bodies (LB) are present in macrophages from the beginning of infection and accumulate in the chronic phase. LB progress from an early (ELB) to a late (LLB) stage, defined according to their progressive capacity to generate cholesterol crystals, resembling atherosclerotic lesions. FM arise from massive accumulation of LLB. Electronic microscopy reveals intracellular lipophilic inclusions (ILIs) in those M. tuberculosis bacilli inside FM. It is our hypothesis that the accumulation of lipids in M. tuberculosis concomitant to the establishment of the non-replicating state prepares the bacilli for future reactivation and for facing future stressful environments. |
| Databáze: | OpenAIRE |
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