Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore
| Autor: | Hua Zhang, Carston R. Wagner, Douglas Yee, Xianke Zeng, Martine Gaillard-Kelly, Qing Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Fluorophore Immunoconjugates Transplantation Heterologous Down-Regulation Mice Nude quantum dots Breast Neoplasms Conjugated system Biology Cell Line Receptor IGF Type 1 03 medical and health sciences chemistry.chemical_compound type I IGF receptor Mice 0302 clinical medicine In vivo Antibody Specificity antibody Cell Line Tumor Animals Humans Receptor 030304 developmental biology Fluorescent Dyes 0303 health sciences tumour imaging Macrophages Antibodies Monoclonal Fibroblasts Fluorescence Small molecule Molecular biology Transplantation body regions Oncology chemistry Quantum dot Fluorescent Antibody Technique Direct 030220 oncology & carcinogenesis Biophysics small-molecule fluorophore Chromatography Gel Female Translational Therapeutics |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Background: The type I insulin-like growth factor receptor (IGF1R) is a transmembrane tyrosine kinase involved in cancer proliferation, survival, and metastasis. Methods: In this study, we used two different fluorescent technologies (small-molecule fluorophores and quantum dot (QD) nanoparticles) to detect receptor expression and its downregulation by antibodies in vivo. Results: After conjugation with AVE-1642, a humanised anti-IGF1R monoclonal antibody, both QDs (705 nm) or Alexa 680 (small-molecule fluorophore) detected expression and downregulation of IGF1R in vitro. To examine their utility in vivo, either AVE-1642 conjugates were intravenously delivered to mice bearing xenograft tumours of mouse embryo fibroblasts expressing human IGF1R or MCF-7 human breast cancer cells. Quantum dot fluorescence was mainly localised to the reticuloendothelial system in several organs and engulfed by macrophages, with only very small amount of QDs detected in the xenograft tumours. Depletion of macrophages by clodronate liposomes did not alter the nonspecific uptake of QDs. In contrast, AVE-1642-conjugated Alexa 680 solely targeted to xenograft tumour and was able to detect IGF1R downregulation, with little nonspecific targeting to other tissues or organs in mice. Conclusion: Taken together, our data suggest that small-molecule fluorophores, not QDs, are suitable to detect the expression and downregulation of IGF1R in vivo. |
| Databáze: | OpenAIRE |
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